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Am J Physiol Renal Physiol 264: F490-F495, 1993;
0363-6127/93 $5.00
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AJP - Renal Physiology, Vol 264, Issue 3 490-F495, Copyright © 1993 by American Physiological Society

ARTICLES

Regulation of high-conductance anion channels by G proteins and 5-HT1A receptors in CHO cells

A. W. Mangel, J. R. Raymond and J. G. Fitz
Division of Gastroenterology, Duke University Medical Center, Durham, North Carolina.

This study addresses the mechanisms responsible for regulation of high-conductance anion channels by GTP binding proteins in Chinese hamster ovary (CHO) cells. Single-channel currents were measured in inside-out membrane patches using patch-clamp techniques. Anion-selective channels with a unitary conductance of 381 +/- 8 pS activated spontaneously in 48% of excised patches. In patches with no spontaneous channel activity, addition of GppNHp, a nonhydrolyzable analogue of GTP, activated channels in 8 of 12 studies, and in patches with spontaneous channel activity, GppNHp increased open probability in 4 of 4 experiments. In contrast, GDP beta S, a nonhydrolyzable GDP analogue, inhibited both spontaneous and GppNHp-induced channel activity. In patches without spontaneous channel activity, addition of cholera toxin activated channels in five of eight studies. Interestingly, pertussis toxin had a similar effect, activating channels in five of seven previously quiescent patches. To further evaluate the possible role of inhibitory G proteins in channel regulation, activity was measured in cell-attached patches in cells transfected with the serotonin 5-HT1A receptor, which is coupled to effector mechanisms through a pertussis toxin-sensitive G protein. Stimulation of 5-HT1A-transfected cells with the receptor agonist (+/-)-8-hydroxy-2-(di-n-propylamino)tetralin caused a transient decrease in open probability in either standard or high-potassium solutions. In aggregate, these findings suggest that both cholera and pertussis toxin-sensitive G proteins contribute to regulation of high-conductance anion channels in CHO cells.


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M. N. Garnovskaya, T. W. Gettys, T. van Biesen, V. Prpic, J. K. Chuprun, and J. R. Raymond
5-HT1A Receptor Activates Na+/H+ Exchange in CHO-K1 Cells through Gialpha 2 and Gialpha 3
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[Abstract] [Full Text] [PDF]


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