AJP - Renal AJP: Renal Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 264: F523-F531, 1993;
0363-6127/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaudies, R. P.
Right arrow Articles by Johnson, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaudies, R. P.
Right arrow Articles by Johnson, J. P.

AJP - Renal Physiology, Vol 264, Issue 3 523-F531, Copyright © 1993 by American Physiological Society

ARTICLES

Increased soluble EGF after ischemia is accompanied by a decrease in membrane-associated precursors

R. P. Schaudies and J. P. Johnson
Department of Nephrology, Walter Reed Army Institute of Research, Washington, District of Columbia 20307.

We have characterized the distribution of immunoreactive epidermal growth factor (irEGF) in control and ischemia-injured rat kidneys. Kidneys that had undergone ischemic injury contained levels of soluble irEGF that were six times those of uninjured kidneys. The predominant forms of soluble irEGF were native and des-Arg-epidermal growth factor (EGF), both of which are biologically active. Crude membrane fractions from whole kidneys were solubilized in Triton X-100 and tested for irEGF. Amounts of irEGF were slightly decreased in the ischemia-injured kidney membranes. However, when solubilized membrane fractions were digested with trypsin, which generates a single immunoreactive species which appears identical to native EGF, the amount of irEGF in control fractions increased 13-fold and the amount in injured fractions increased only 4-fold as measured by radioimmunoassay. To better characterize the membrane-associated irEGF, Triton X-100-solubilized membrane fractions from control animals were affinity purified and subjected to high-performance liquid molecular sieve chromatography. Three major peaks of material exhibited immunoreactivity to EGF antibodies, bound the EGF receptor, and stimulated [3H]thymidine incorporation in growth-arrested fibroblasts. Trypsin digestion of the two high-molecular-mass peaks enhanced these activities. The third peak eluted with native EGF and showed no change in activity with trypsin addition. We propose that EGF is released from membrane-associated EGF precursors and can then act in an autocrine or paracrine fashion to promote cell growth after ischemia-induced acute renal failure.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Marechal, H. Jammes, B. Rossignol, and P. Mauduit
EGF precursor mRNA and membrane-associated EGF precursor protein in rat exorbital lacrimal gland
Am J Physiol Cell Physiol, March 1, 1999; 276(3): C734 - C746.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. B. Keasler, D. E. McKeller, and R. G. Schnellmann
Differential effects of EGF on repair of cellular functions after dichlorovinyl-L-cysteine-induced injury
Am J Physiol Renal Physiol, February 1, 1999; 276(2): F228 - F236.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Z. Symon, S. Fuchs, Y. Agmon, O. Weiss, I. Nephesh, R. Moshe, M. Brezis, A. Flyvbjerg, and I. Raz
The endogenous insulin-like growth factor system in radiocontrast nephropathy
Am J Physiol Renal Physiol, March 1, 1998; 274(3): F490 - F497.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online