| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 264, Issue 5 781-F791, Copyright © 1993 by American Physiological Society
ARTICLES |
K. M. Todd-Turla, J. Schnermann, G. Fejes-Toth, A. Naray-Fejes-Toth, A. Smart, P. D. Killen and J. P. Briggs
Department of Physiology, University of Michigan, Ann Arbor 48109.
In the present study, a competitive polymerase chain reaction (PCR) technique was used to quantitate the relative levels of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) mRNA in microdissected nephron segments from the rat kidney and of MR mRNA from isolated principal and intercalated collecting duct cells from rabbit. RNA was isolated from cells and isolated tubules, cDNA was synthesized, and receptor cDNA was coamplified by PCR with a competitive control template. beta-Actin PCR products were also obtained from each nephron segment studied, to assess variations in RNA extraction and cDNA synthesis. MR mRNA, as determined by this competitive PCR technique, was 10-fold more abundant in cortical collecting duct (CCD), outer medullary collecting duct, and inner medullary collecting duct segments than in the proximal tubule and thick ascending limb segments (P < 0.05). Both principal and beta-intercalated cells of the CCD contained detectable levels of MR mRNA, although the levels in the principal cells were threefold higher (P < 0.01). GR mRNA was twofold more abundant in glomeruli, proximal tubule, and thick ascending limb segments than in the collecting duct segments (P < 0.05). In general, the distribution pattern of MR and GR mRNA is consistent with the distribution of adrenal corticosteroid function along the nephron.
This article has been cited by other articles:
|
|
 |
|
  D. C. A. Leite-Dellova, M. Oliveira-Souza, G. Malnic, and M. Mello-Aires Genomic and nongenomic dose-dependent biphasic effect of aldosterone on Na+/H+ exchanger in proximal S3 segment: role of cytosolic calcium Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1342 - F1352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Martel, D. Michael, G. Fejes-Toth, and A. Naray-Fejes-Toth Melanophilin, a novel aldosterone-induced gene in mouse cortical collecting duct cells Am J Physiol Renal Physiol, September 1, 2007; 293(3): F904 - F913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Otani, F. Otsuka, K. Inagaki, M. Takeda, T. Miyoshi, J. Suzuki, T. Mukai, T. Ogura, and H. Makino Antagonistic effects of bone morphogenetic protein-4 and -7 on renal mesangial cell proliferation induced by aldosterone through MAPK activation Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1513 - F1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Perez-Rojas, S. Derive, J. A. Blanco, C. Cruz, L. M. de la Maza, G. Gamba, and N. A. Bobadilla Renocortical mRNA expression of vasoactive factors during spironolactone protective effect in chronic cyclosporine nephrotoxicity Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1020 - F1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Rad, R. J. Balment, and N. Ashton Rapid natriuretic action of aldosterone in the rat J Appl Physiol, February 1, 2005; 98(2): 423 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gonzalez-Nunez, M. Morales-Ruiz, A. Leivas, S. C. Hebert, and E. Poch In vitro characterization of aldosterone and cAMP effects in mouse distal convoluted tubule cells Am J Physiol Renal Physiol, May 1, 2004; 286(5): F936 - F944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Madsen, J. Stubbe, T. Yang, O. Skott, S. Bachmann, and B. L. Jensen Low endogenous glucocorticoid allows induction of kidney cortical cyclooxygenase-2 during postnatal rat development Am J Physiol Renal Physiol, January 1, 2004; 286(1): F26 - F37. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, T. R. Uhrenholt, C. Bistrup, O. Skott, and B. L. Jensen Stimulation of 11-{beta}-hydroxysteroid dehydrogenase type 2 in rat colon but not in kidney by low dietary NaCl intake Am J Physiol Renal Physiol, August 1, 2003; 285(2): F348 - F358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Helms, G. Fejes-Toth, and A. Naray-Fejes-Toth Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression Am J Physiol Renal Physiol, March 1, 2003; 284(3): F480 - F487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Good, T. George, and B. A. Watts III Aldosterone inhibits HCO-3 absorption via a nongenomic pathway in medullary thick ascending limb Am J Physiol Renal Physiol, October 1, 2002; 283(4): F699 - F706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kretzler, C. D. Cohen, P. Doran, A. Henger, S. Madden, E. F. Grone, P. J. Nelson, D. Schlondorff, and H.-J. Grone Repuncturing the Renal Biopsy: Strategies for Molecular Diagnosis in Nephrology J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1961 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Farman and M.-E. Rafestin-Oblin Multiple aspects of mineralocorticoid selectivity Am J Physiol Renal Physiol, February 1, 2001; 280(2): F181 - F192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Feraille and A. Doucet Sodium-Potassium-Adenosinetriphosphatase-Dependent Sodium Transport in the Kidney: Hormonal Control Physiol Rev, January 1, 2001; 81(1): 345 - 418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Reilly and D. H. Ellison Mammalian Distal Tubule: Physiology, Pathophysiology, and Molecular Anatomy Physiol Rev, January 1, 2000; 80(1): 277 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Naray-Fejes-Toth, C. Canessa, E. S. Cleaveland, G. Aldrich, and G. Fejes-Toth sgk Is an Aldosterone-induced Kinase in the Renal Collecting Duct. EFFECTS ON EPITHELIAL Na+ CHANNELS J. Biol. Chem., June 11, 1999; 274(24): 16973 - 16978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. BENS, V. VALLET, F. CLUZEAUD, L. PASCUAL-LETALLEC, A. KAHN, M. E. RAFESTIN-OBLIN, B. C. ROSSIER, and A. VANDEWALLE Corticosteroid-Dependent Sodium Transport in a Novel Immortalized Mouse Collecting Duct Principal Cell Line J. Am. Soc. Nephrol., May 1, 1999; 10(5): 923 - 934. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Guo, L. Liu, and L. Barajas RT-PCR study of the distribution of connexin 43 mRNA in the glomerulus and renal tubular segments Am J Physiol Regulatory Integrative Comp Physiol, August 1, 1998; 275(2): R439 - R447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Husted, J. P. Rapp, and J. B. Stokes Candidate Genes in the Regulation of Na+ Transport by Inner Medullary Collecting Duct Cells From Dahl Rats Hypertension, February 1, 1998; 31(2): 608 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yang, S. A. Hassan, I. Singh, A. Smart, F. C. Brosius, L. B. Holzman, J. B. Schnermann, and J. P. Briggs SA Gene Expression in the Proximal Tubule of Normotensive and Hypertensive Rats Hypertension, March 1, 1996; 27(3): 541 - 545. [Abstract] [Full Text]
|
|
|
|
|
|
A. Attmane-Elakeb, V. Sibella, C. Vernimmen, X. Belenfant, S. C. Hebert, and M. Bichara Regulation by Glucocorticoids of Expression and Activity of rBSC1, the Na+-K+(NH4+)-2Cl- Cotransporter of Medullary Thick Ascending Limb J. Biol. Chem., October 20, 2000; 275(43): 33548 - 33553. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
