AJP - Renal Physiology, Vol 265, Issue 1 146-F150, Copyright © 1993 by American Physiological Society

ARTICLES

Developmentally regulated kallikrein enzymatic activity and gene transcription rate in maturing rat kidneys

S. S. el-Dahr and I. Yosipiv
Department of Pediatrics, Tulane University School of Medicine, New Orleans, Louisiana 70112.

Kinins are paracrine vasoactive and growth-modulating peptides. Kidney maturation is accompanied by enhanced accumulation of the mRNA encoding tissue (renal) kallikrein, a serine protease, and a key component of the kallikrein-kinin system. To further delineate the developmental regulation of renal kallikrein, we measured tissue kallikrein activity and gene transcription rate during the latter stages of metanephrogenesis. Active tissue kallikrein was measured in renal homogenates by the amidolytic assay using the fluorogenic substrate D-Val-Leu-Arg-7-amino-4-methylcoumarin (D-VLR-AMC) in the presence or absence of soybean trypsin inhibitor (SBTI). Kallikrein activity was detectable at very low levels in the near-term fetal metanephros. Postnatally, renal kallikrein activity peaked immediately after birth and again at the time of weaning (P < 0.05 vs. other age groups) and remained high in the adult. Mature female rat kidneys contained 30% more active kallikrein than male kidneys (P < 0.05). The SBTI-sensitive D-VLR-AMC hydrolytic activity (due to serine proteases other than tissue kallikrein) accounted for 36-53% of the total renal amidolytic activity. Compared with the 5-day-old newborn, steady-state renal kallikrein mRNA levels increased threefold by day 12 and sixfold by adulthood. Run-on transcription analysis of renal cell nuclei revealed a significant increase in kallikrein gene transcription rate of 80% on day 12 (P < 0.05) and 480% in the adult (P < 0.001). The presence of active kallikrein in the developing kidney and the upregulation of its synthesis at specific time points during postnatal development implicate intrarenal kinins as potential modulators of renal growth and functional maturation.(ABSTRACT TRUNCATED AT 250 WORDS)

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