| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 265, Issue 2 204-F213, Copyright © 1993 by American Physiological Society
ARTICLES |
S. Nielsen and M. A. Knepper
Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
The present studies were performed to investigate the kinetics of regulation of water channels and urea carriers in the rat terminal (IMCD) in response to vasopressin (AVP). The time courses of osmotic water permeability (Pf) and urea permeability (P(urea)) were measured in isolated perfused rat terminal IMCD segments following AVP stimulation and subsequently following AVP washout. Under control conditions, Pf and P(urea) kinetics were similar. Both transport processes exhibited complex patterns of activation with a period of rapid permeability increase followed by a period of slower increase. Both transporters also exhibited complex patterns of reversal following AVP washout, with a rapid permeability decrease (5 min) followed by a slower decrease toward the baseline value. The measurements were repeated in the presence of a lumen > bath osmotic gradient, a condition associated with a decreased rate of apical endocytosis in collecting ducts. The lumen > bath gradient did not alter the kinetics of Pf increase after AVP addition, but completely blocked the decrease in Pf normally seen with washout of AVP. In contrast, the lumen > bath osmotic gradient did not affect the decrease in urea permeability after AVP washout, but blocked the rapid phase of urea permeability increase following AVP addition. Thus imposition of a lumen > bath osmotic gradient resulted in separation of the time courses of P(urea) and Pf changes associated with AVP addition and washout. This finding indicates that the physical processes responsible for AVP-mediated alteration of urea transporter and water channel activity in the apical membrane are distinct.
This article has been cited by other articles:
|
|
 |
|
  N. W. Blessing, M. A. Blount, J. M. Sands, C. F. Martin, and J. D. Klein Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1336 - F1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chen, H. Huang, O. Frohlich, Y. Yang, J. D. Klein, S. R. Price, and J. M. Sands MDM2 E3 ubiquitin ligase mediates UT-A1 urea transporter ubiquitination and degradation Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1528 - F1534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-J. Yu, T. Pisitkun, G. Wang, J. F. Aranda, P. A. Gonzales, D. Tchapyjnikov, R.-F. Shen, M. A. Alonso, and M. A. Knepper Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct Am J Physiol Cell Physiol, September 1, 2008; 295(3): C661 - C678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Klein, O. Frohlich, M. A. Blount, C. F. Martin, T. D. Smith, and J. M. Sands Vasopressin Increases Plasma Membrane Accumulation of Urea Transporter UT-A1 in Rat Inner Medullary Collecting Ducts J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2680 - 2686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-W. Lim, K.-H. Han, J.-Y. Jung, W.-Y. Kim, C.-W. Yang, J. M. Sands, M. A. Knepper, K. M. Madsen, and J. Kim Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R479 - R492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Frohlich, J. D. Klein, P. M. Smith, J. M. Sands, and R. B. Gunn Urea transport in MDCK cells that are stably transfected with UT-A1 Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1264 - C1270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sands Molecular Approaches to Urea Transporters J. Am. Soc. Nephrol., November 1, 2002; 13(11): 2795 - 2806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Holmes, B. M. Patel, J. A. Russell, and K. R. Walley Physiology of Vasopressin Relevant to Management of Septic Shock Chest, September 1, 2001; 120(3): 989 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nakamura, T. Hirano, K. Tsujimae, M. Aoyama, K. Kondo, Y. Yamamura, T. Mori, and M. Tominaga Antidiuretic Effects of a Nonpeptide Vasopressin V2-Receptor Agonist, OPC-51803, Administered Orally to Rats J. Pharmacol. Exp. Ther., December 1, 2000; 295(3): 1005 - 1011. [Abstract] [Full Text]
|
|
|
|
|
|
T. Inoue, J. Terris, C. A. Ecelbarger, C.-L. Chou, S. Nielsen, and M. A. Knepper Vasopressin regulates apical targeting of aquaporin-2 but not of UT1 urea transporter in renal collecting duct Am J Physiol Renal Physiol, April 1, 1999; 276(4): F559 - F566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. SANDS Regulation of Renal Urea Transporters J. Am. Soc. Nephrol., March 1, 1999; 10(3): 635 - 646. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kato and J. M. Sands Urea transport processes are induced in rat IMCD subsegments when urine concentrating ability is reduced Am J Physiol Renal Physiol, January 1, 1999; 276(1): F62 - F71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Christensen, D. Marples, U. B. Jensen, J. Frokiaer, D. Sheikh-Hamad, M. Knepper, and S. Nielsen Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution Am J Physiol Renal Physiol, August 1, 1998; 275(2): F285 - F297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Kishore, J. B. Wade, K. Schorr, T. Inoue, B. Mandon, and M. A. Knepper Expression of synaptotagmin VIII in rat kidney Am J Physiol Renal Physiol, July 1, 1998; 275(1): F131 - F142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Park, G. Koike, and A. W. Cowley Jr. Regional time-dependent changes in vasopressin V2 receptor expression in the rat kidney during water restriction Am J Physiol Renal Physiol, May 1, 1998; 274(5): F906 - F913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sands, F. X. Flores, A. Kato, M. A. Baum, E. M. Brown, D. T. Ward, S. C. Hebert, and H. W. Harris Vasopressin-elicited water and urea permeabilities are altered in IMCD in hypercalcemic rats Am J Physiol Renal Physiol, May 1, 1998; 274(5): F978 - F985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Chou, T. Ma, B. Yang, M. A. Knepper, and A. S. Verkman Fourfold reduction of water permeability in inner medullary collecting duct of aquaporin-4 knockout mice Am J Physiol Cell Physiol, February 1, 1998; 274(2): C549 - C554. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A.S. Santos, A. C. Simoes e Silva, A. J. Magaldi, M. C. Khosla, K. R. Cesar, K. T. Passaglio, and N. C.V. Baracho Evidence for a Physiological Role of Angiotensin-(1-7) in the Control of Hydroelectrolyte Balance Hypertension, April 1, 1996; 27(4): 875 - 884. [Abstract] [Full Text]
|
|
|
|
|
|
C. Zhang, J. M. Sands, and J. D. Klein Vasopressin rapidly increases phosphorylation of UT-A1 urea transporter in rat IMCDs through PKA Am J Physiol Renal Physiol, January 1, 2002; 282(1): F85 - F90. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
