| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 265, Issue 4 598-F603, Copyright © 1993 by American Physiological Society
ARTICLES |
P. Kofuji, W. J. Lederer and D. H. Schulze
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
The cardiac (versus retinal rod) Na/Ca exchanger gene has been cloned, sequenced and shown by RNA analysis to be present in diverse tissues. Analysis of published sequences shows that a single isoform is found in heart tissue from many species (NACA1 isoform). We provide evidence here by ribonuclease (RNase) protection assays and by reverse transcriptase-polymerase chain reaction (PCR) amplification with sequence analysis that a new isoform encoding the Na/Ca exchanger is present in renal tissue. This isoform (NACA3) reveals a 7-amino acid deletion in the tested region compared with the NACA2 isoform described by Reilly and Shugrue [Am. J. Physiol. 262 (Renal Fluid Electrolyte Physiol. 31): F1105-F1109, 1992] and is the dominant exchanger transcript in kidney. Analysis of the sequence of all isoforms indicates that the differences in the isoforms reside in the large intracellular loop region of the protein. Alternative splicing of a single Na/Ca exchanger message may be responsible for these tissue-specific transcripts.
This article has been cited by other articles:
|
|
 |
|
  F. Schweda, H. Seebauer, B. K. Kramer, and A. Kurtz Functional role of sodium-calcium exchange in the regulation of renal vascular resistance Am J Physiol Renal Physiol, January 1, 2001; 280(1): F155 - F161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Unlap, J. Peti-Peterdi, and P. D. Bell Cloning of mesangial cell Na+/Ca2+ exchangers from Dahl/Rapp salt-sensitive/resistant rats Am J Physiol Renal Physiol, July 1, 2000; 279(1): F177 - F184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. F. Reilly and D. H. Ellison Mammalian Distal Tubule: Physiology, Pathophysiology, and Molecular Anatomy Physiol Rev, January 1, 2000; 80(1): 277 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Blaustein and W. J. Lederer Sodium/Calcium Exchange: Its Physiological Implications Physiol Rev, July 1, 1999; 79(3): 763 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cheng, T. P. Hagen, M. L. Dawson, K. V. Barnes, and D. R. Menick The Role of GATA, CArG, E-box, and a Novel Element in the Regulation of Cardiac Expression of the Na+-Ca2+ Exchanger Gene J. Biol. Chem., April 30, 1999; 274(18): 12819 - 12826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Nelson, M. T. Unlap, J. L. Lewis, and P. D. Bell Renal arteriolar Na+/Ca2+ exchange in salt-sensitive hypertension Am J Physiol Renal Physiol, April 1, 1999; 276(4): F567 - F573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Mashburn, M. T. Unlap, J. Runquist, A. Alderman, G. V. W. Johnson, and P. D. Bell Altered protein kinase C activation of Na+/Ca2+ exchange in mesangial cells from salt-sensitive rats Am J Physiol Renal Physiol, April 1, 1999; 276(4): F574 - F580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Shuba, T. Iwata, V. G. Naidenov, M. Oz, K. Sandberg, A. Kraev, E. Carafoli, and M. Morad A Novel Molecular Determinant for cAMP-dependent Regulation of the Frog Heart Na+-Ca2+ Exchanger J. Biol. Chem., July 24, 1998; 273(30): 18819 - 18825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. He, A. Ruknudin, L. L. Bambrick, W. J. Lederer, and D. H. Schulze Isoform-Specific Regulation of the Na+/Ca2+ Exchanger in Rat Astrocytes and Neurons by PKA J. Neurosci., July 1, 1998; 18(13): 4833 - 4841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakamura, S. Arii, K. Monden, M. Furutani, Y. Takeda, M. Imamura, M. Tominaga, and Y. Okada Expression of the Na+/Ca2+ exchanger emerges in hepatic stellate cells after activation in association with liver fibrosis PNAS, April 28, 1998; 95(9): 5389 - 5394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Nicholas, W. Yang, S.-L. Lee, H. Zhu, K. D. Philipson, and J. Lytton Alternative promoters and cardiac muscle cell-specific expression of the Na+/Ca2+ exchanger gene Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H217 - H232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. V. Barnes, G. Cheng, M. M. Dawson, and D. R. Menick Cloning of Cardiac, Kidney, and Brain Promoters of the Feline ncx1 Gene J. Biol. Chem., April 25, 1997; 272(17): 11510 - 11517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Muller, Y. Isomatsu, S. V. Koushik, M. O'Quinn, L. Xu, C. S. Kappler, E. Hapke, M. R. Zile, S. J. Conway, and D. R. Menick Cardiac-Specific Expression and Hypertrophic Upregulation of the Feline Na+-Ca2+ Exchanger Gene H1-Promoter in a Transgenic Mouse Model Circ. Res., February 8, 2002; 90(2): 158 - 164. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
