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Am J Physiol Renal Physiol 266: F298-F308, 1994;
0363-6127/94 $5.00
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AJP - Renal Physiology, Vol 266, Issue 2 298-F308, Copyright © 1994 by American Physiological Society

ARTICLES

Growth and characterization of porcine urinary bladder epithelial cells in vitro

C. Guhe and W. Follmann
Institut fur Arbeitsphysiologie, Universitat Dortmund, Germany.

Dividing long-term monolayer cultures of porcine urinary bladder epithelial cells were obtained by a combined mechanical and enzymatic isolation method. The serum-free cultured cells were investigated morphologically and characterized according to their growth characteristics and enzymatic functions. Investigations over a period up to 12 wk demonstrated that the cells regain their in vivo polarization with apically situated membrane vesicles and tight junctions between neighboring cells when they have built up a confluent monolayer. Activities of most marker enzymes for cell-differentiated status and function of such cells observed over a period of 4 wk in culture were conserved compared with the original tissue. Lactate dehydrogenase activity release into the medium was at low levels (< or = 5% of the total amount), indicating a good membrane integrity and cell viability. The chromosome set (2n = 38) did not change significantly during the first 5 wk, but, with additional culture time, the degree of polyploid and polynucleated cells increased comparable to the in vivo situation, in which the apical cell layer of the bladder mucosa also showed a high degree of polynucleation and polyploidy, indicative of a senescence process.


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