AJP - Renal Physiology, Vol 266, Issue 3 491-F496, Copyright © 1994 by American Physiological Society

ARTICLES

Synthesis and localization of C-type natriuretic peptide in mammalian kidney

A. D. Dean, V. M. Vehaskari and J. E. Greenwald
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.

We have previously demonstrated the synthesis of atrial natriuretic factor (ANF) in the distal cortical nephron of the rat kidney. We now report the synthesis of C-type natriuretic peptide (CNP) in the rat, mouse, and human nephron. CNP mRNA was initially detected in the rat and mouse kidney, as well as in three transformed cell lines isolated from the proximal and distal nephron, using reverse transcription-polymerase chain reaction (RT-PCR). Confirmation of the kidney PCR product was performed by nucleotide sequence analysis and Southern hybridization. Northern hybridization of rat brain CNP cDNA to human kidney polyadenylated RNA detected a 1.4-kb gene transcript. Rat nephron segments were microdissected and subjected to RT-PCR to localize CNP mRNA. CNP mRNA was detected in the proximal convoluted tubule, cortical collecting duct, medullary thick limbs, and inner medullary collecting ducts. CNP, as detected by immunohistochemistry, was found to colocalize with CNP mRNA.

This article has been cited by other articles:

				A. Cataliotti, M. Giordano, E. De Pascale, G. Giordano, P. Castellino, M. Jougasaki, L. C. Costello, G. Boerrigter, T. Tsuruda, P. Belluardo, et al. CNP production in the kidney and effects of protein intake restriction in nephrotic syndrome Am J Physiol Renal Physiol, September 1, 2002; 283(3): F464 - F472. [Abstract] [Full Text] [PDF]

				F. El-Gehani, M. Tena-Sempere, H. Ruskoaho, and I. Huhtaniemi Natriuretic Peptides Stimulate Steroidogenesis in the Fetal Rat Testis Biol Reprod, August 1, 2001; 65(2): 595 - 600. [Abstract] [Full Text] [PDF]

				V Gulberg, S Moller, J H Henriksen, and A L Gerbes Increased renal production of C-type natriuretic peptide (CNP) in patients with cirrhosis and functional renal failure Gut, December 1, 2000; 47(6): 852 - 857. [Abstract] [Full Text] [PDF]

				M. KASAHARA, M. MUKOYAMA, A. SUGAWARA, H. MAKINO, T. SUGANAMI, Y. OGAWA, M. NAKAGAWA, K. YAHATA, M. GOTO, R. ISHIBASHI, et al. Ameliorated Glomerular Injury in Mice Overexpressing Brain Natriuretic Peptide with Renal Ablation J. Am. Soc. Nephrol., September 1, 2000; 11(9): 1691 - 1701. [Abstract] [Full Text]

				J. R. Klinger, F. M. Siddiq, R. A. Swift, C. Jackson, L. Pietras, R. R. Warburton, C. Alia, and N. S. Hill C-type natriuretic peptide expression and pulmonary vasodilation in hypoxia-adapted rats Am J Physiol Lung Cell Mol Physiol, October 1, 1998; 275(4): L645 - L652. [Abstract] [Full Text] [PDF]

				I. Pham, S. Sediame, G. Maistre, F. Roudot-Thoraval, P.-E. Chabrier, A. Carayon, and S. Adnot Renal and vascular effects of C-type and atrial natriuretic peptides in humans Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1997; 273(4): R1457 - R1464. [Abstract] [Full Text] [PDF]