AJP - Renal Physiology, Vol 267, Issue 1 44-F48, Copyright © 1994 by American Physiological Society

ARTICLES

Prostaglandins mediate the defect in AVP-stimulated cAMP generation in immature collecting duct

M. Bonilla-Felix and C. John-Phillip
Department of Pediatrics, University of Texas Health Science Center at Houston 77030.

The immature kidney is characterized by resistance to arginine vasopressin (AVP). In the immature cortical collecting duct (iCCD), AVP-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) generation is decreased, but the mechanisms involved are not known. We examined cAMP production in isolated CCD from immature and mature rabbits. Cellular cAMP levels were measured by radioimmunoassay under basal conditions and after stimulation with hormone. Basal cAMP production in the iCCD was not different from that in the mature CCD (mCCD). In contrast, AVP- and forskolin-stimulated cAMP generation were severely decreased in the iCCD. Inhibition of endogenous prostaglandin production by indomethacin increased AVP-stimulated cAMP generation in the iCCD to levels that were not different from the mCCD. Inhibition of protein kinase C (PKC) by staurosporine and inhibition of Gi by pertussis toxin elicited a mature cAMP response in the iCCD. These data suggest that the defect in AVP-stimulated cAMP production in the iCCD is mediated by prostaglandins via 1) activation of Gi and 2) direct inhibition of the adenylyl cyclase catalytic subunit. In addition, PKC appears to play a significant role.

This article has been cited by other articles:

				M. Bonilla-Felix Development of water transport in the collecting duct Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1093 - F1101. [Abstract] [Full Text] [PDF]

				R. Quigley, S. Chakravarty, and M. Baum Antidiuretic hormone resistance in the neonatal cortical collecting tubule is mediated in part by elevated phosphodiesterase activity Am J Physiol Renal Physiol, February 1, 2004; 286(2): F317 - F322. [Abstract] [Full Text] [PDF]