AJP - Renal Physiology, Vol 267, Issue 2 215-F222, Copyright © 1994 by American Physiological Society

ARTICLES

PCR localization of C-type natriuretic peptide and B-type receptor mRNAs in rat nephron segments

Y. Terada, K. Tomita, H. Nonoguchi, T. Yang and F. Marumo
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

The present study was undertaken to investigate the presence of C-type natriuretic peptide (CNP) mRNA and its receptor, natriuretic peptide B-type receptor (ANPR-B) mRNA, in rat renal structures. The microlocalization of mRNAs coding for CNP and ANPR-B was carried out in the rat kidney, using an assay of reverse transcription and polymerase chain reaction (RT-PCR) in individual microdissected renal tubule segments, glomeruli, vasa recta bundle, and arcuate arteries. The PCR signal for CNP was detected in glomerulus, vasa recta bundle, and arcuate artery. The PCR product of ANPR-B was widely present in renal structures. Relatively large amounts of ANPR-B PCR product were detected in glomerulus, vasa recta bundle, arcuate artery, and distal nephron segments. A relatively high concentration of CNP (10(-7) M) stimulated guanosine 3',5'-cyclic monophosphate accumulation in glomerulus, medullary thick ascending limb, cortical collecting duct, and inner medullary collecting duct. Our data demonstrate that CNP can be produced locally in the glomerulus and renal vascular system and that ANPR-B is widely distributed in renal structures. Thus CNP may influence renal function and act in autocrine and paracrine fashions in the kidney.

This article has been cited by other articles:

				Y. Takei, K. Inoue, K. Ando, T. Ihara, T. Katafuchi, M. Kashiwagi, and S. Hirose Enhanced expression and release of C-type natriuretic peptide in freshwater eels Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1727 - R1735. [Abstract] [Full Text] [PDF]

				S. Hanada, Y. Terada, S. Inoshita, S. Sasaki, S. M. Lohmann, A. Smolenski, and F. Marumo Overexpression of protein kinase G using adenovirus inhibits cyclin E transcription and mesangial cell cycle Am J Physiol Renal Physiol, May 1, 2001; 280(5): F851 - F859. [Abstract] [Full Text] [PDF]

				V Gulberg, S Moller, J H Henriksen, and A L Gerbes Increased renal production of C-type natriuretic peptide (CNP) in patients with cirrhosis and functional renal failure Gut, December 1, 2000; 47(6): 852 - 857. [Abstract] [Full Text] [PDF]

				M. Oliveira-Souza and M. De Mello-Aires Interaction of angiotensin II and atrial natriuretic peptide on pHi regulation in MDCK cells Am J Physiol Renal Physiol, November 1, 2000; 279(5): F944 - F953. [Abstract] [Full Text] [PDF]

				J. R. HIRSCH, M. MEYER, H.-J. MÄGERT, W.-G. FORSSMANN, S. MOLLERUP, P. HERTER, G. WEBER, R. CERMAK, I. ANKORINA-STARK, E. SCHLATTER, et al. cGMP-Dependent and -Independent Inhibition of a K+ Conductance by Natriuretic Peptides: Molecular and Functional Studies in Human ProximalTubule Cells J. Am. Soc. Nephrol., March 1, 1999; 10(3): 472 - 480. [Abstract] [Full Text]

				L. Cao, S. C. Chen, T. Cheng, M. H. Humphreys, and D. G. Gardner Ligand-dependent regulation of NPR-A gene expression in inner medullary collecting duct cells Am J Physiol Renal Physiol, July 1, 1998; 275(1): F119 - F125. [Abstract] [Full Text] [PDF]

				G. Barletta, C. Lazzeri, S. Vecchiarino, R. Del Bene, G. Messeri, A. Dello Sbarba, M. Mannelli, and G. La Villa Low-Dose C-Type Natriuretic Peptide Does Not Affect Cardiac and Renal Function in Humans Hypertension, March 1, 1998; 31(3): 802 - 808. [Abstract] [Full Text] [PDF]

				P.-L. Tharaux, J.-C. Dussaule, S. Couette, and C. Clerici Evidence for functional ANP receptors in cultured alveolar type II cells Am J Physiol Lung Cell Mol Physiol, February 1, 1998; 274(2): L244 - L251. [Abstract] [Full Text] [PDF]

				J. Amin, O. A. Carretero, and S. Ito Mechanisms of Action of Atrial Natriuretic Factor and C-Type Natriuretic Peptide Hypertension, March 1, 1996; 27(3): 684 - 687. [Abstract] [Full Text]