AJP - Renal Physiology, Vol 267, Issue 2 289-F295, Copyright © 1994 by American Physiological Society

ARTICLES

ATP inhibits the hydrosmotic effect of AVP in rabbit CCT: evidence for a nucleotide P2u receptor

D. Rouse, M. Leite and W. N. Suki
Department of Medicine, Baylor College of Medicine, Houston, Texas.

In rabbit renal cortical collecting tubule (CCT), perfused in vitro at 38 degrees C, ATP in concentrations of 10(-7) M and greater inhibits arginine vasopressin (AVP)-stimulated osmotic water permeability (Pf). The P1-purinergic receptor antagonist 8-phenyltheophylline did not attenuate the inhibitory action of ATP, and the poorly hydrolyzable ATP analogue, 5'-adenylylimidodiphosphate (AMP-PNP), mimicked the effect of ATP, arguing against an effect of ATP on a P1 receptor or the "P site." Purinergic receptor agonists inhibited AVP-stimulated Pf with the following rank order efficacy: ATP = ADP = UTP = AMP-PNP = alpha, beta-methylene-ATP > 2-methylthio-ATP >> AMP > adenosine, consistent with the pharmacology of a "nucleotide" receptor subtype. Pertussis toxin pretreatment attenuated the action of 10(-5) and 10(-6) MATP; however, 10(-4) MATP failed to inhibit the hydrosmotic action of forskolin or 8-bromoadenosine 3',5'-cyclic monophosphate. Pretreatment with the phosphodiesterase inhibitor RO20-1724 or indomethacin did not inhibit the action of ATP. Staurosporin and 3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester significantly attenuated the inhibition of Pf by lower concentrations of ATP. These data suggest that ATP activates nucleotide receptors on the CCT, mobilizing intracellular Ca2+, which inhibits the hydrosmotic action of AVP.

This article has been cited by other articles:

				Y. Zhang, J. M. Sands, D. E. Kohan, R. D. Nelson, C. F. Martin, N. G. Carlson, C. D. Kamerath, Y. Ge, J. D. Klein, and B. K. Kishore Potential role of purinergic signaling in urinary concentration in inner medulla: insights from P2Y2 receptor gene knockout mice Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1715 - F1724. [Abstract] [Full Text] [PDF]

				V. Vallon P2 receptors in the regulation of renal transport mechanisms Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27. [Abstract] [Full Text] [PDF]

				D. G. Shirley, M. A. Bailey, and R. J. Unwin In vivo stimulation of apical P2 receptors in collecting ducts: evidence for inhibition of sodium reabsorption Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1243 - F1248. [Abstract] [Full Text] [PDF]

				J. Leipziger Control of epithelial transport via luminal P2 receptors Am J Physiol Renal Physiol, March 1, 2003; 284(3): F419 - F432. [Abstract] [Full Text] [PDF]

				C. B. Woda, M. Leite Jr., R. Rohatgi, and L. M. Satlin Effects of luminal flow and nucleotides on [Ca2+]i in rabbit cortical collecting duct Am J Physiol Renal Physiol, September 1, 2002; 283(3): F437 - F446. [Abstract] [Full Text] [PDF]

				M. Zelenina, S. Zelenin, A. A. Bondar, H. Brismar, and A. Aperia Water permeability of aquaporin-4 is decreased by protein kinase C and dopamine Am J Physiol Renal Physiol, August 1, 2002; 283(2): F309 - F318. [Abstract] [Full Text] [PDF]

				H. Lehrmann, J. Thomas, S. J. Kim, C. Jacobi, and J. Leipziger Luminal P2Y2 Receptor-Mediated Inhibition of Na+ Absorption in Isolated Perfused Mouse CCD J. Am. Soc. Nephrol., January 1, 2002; 13(1): 10 - 18. [Abstract] [Full Text] [PDF]

				L.-J. Dai, H. S. Kang, D. Kerstan, G. Ritchie, and G. A. Quamme ATP inhibits Mg2+ uptake in MDCT cells via P2X purinoceptors Am J Physiol Renal Physiol, November 1, 2001; 281(5): F833 - F840. [Abstract] [Full Text] [PDF]

				E. M. Schwiebert and B. K. Kishore Extracellular nucleotide signaling along the renal epithelium Am J Physiol Renal Physiol, June 1, 2001; 280(6): F945 - F963. [Abstract] [Full Text] [PDF]

				P. DEETJEN, J. THOMAS, H. LEHRMANN, S. J. KIM, and J. LEIPZIGER The Luminal P2Y Receptor in the Isolated Perfused Mouse Cortical Collecting Duct J. Am. Soc. Nephrol., October 1, 2000; 11(10): 1798 - 1806. [Abstract] [Full Text]

				M. Bidet, G. De Renzis, S. Martial, I. Rubera, M. Tauc, and P. Poujeol Extracellular ATP increases [CA2+]i in distal tubule cells. I. Evidence for a P2Y2 purinoceptor Am J Physiol Renal Physiol, July 1, 2000; 279(1): F92 - F101. [Abstract] [Full Text] [PDF]

				J. G. J. Hoenderop, P. H. G. M. Willems, and R. J. M. Bindels Toward a comprehensive molecular model of active calcium reabsorption Am J Physiol Renal Physiol, March 1, 2000; 278(3): F352 - F360. [Abstract] [Full Text] [PDF]

				B. K. Kishore, S. M. Ginns, C. M. Krane, S. Nielsen, and M. A. Knepper Cellular localization of P2Y2 purinoceptor in rat renal inner medulla and lung Am J Physiol Renal Physiol, January 1, 2000; 278(1): F43 - F51. [Abstract] [Full Text] [PDF]

				J. van Baal, J. G. J. Hoenderop, M. Groenendijk, C. H. van Os, R. J. M. Bindels, and P. H. G. M. Willems Hormone-stimulated Ca2+ transport in rabbit kidney: multiple sites of inhibition by exogenous ATP Am J Physiol Renal Physiol, December 1, 1999; 277(6): F899 - F906. [Abstract] [Full Text] [PDF]

				D. E. McCoy, A. L. Taylor, B. A. Kudlow, K. Karlson, M. J. Slattery, L. M. Schwiebert, E. M. Schwiebert, and B. A. Stanton Nucleotides regulate NaCl transport in mIMCD-K2 cells via P2X and P2Y purinergic receptors Am J Physiol Renal Physiol, October 1, 1999; 277(4): F552 - F559. [Abstract] [Full Text] [PDF]

				J.-S. Zheng, M. O. Boluyt, X. Long, L. O'Neill, E. G. Lakatta, and M. T. Crow Extracellular ATP Inhibits Adrenergic Agonist–Induced Hypertrophy of Neonatal Cardiac Myocytes Circ. Res., April 1, 1996; 78(4): 525 - 535. [Abstract] [Full Text]

				E. M. Schwiebert, D. P. Wallace, G. M. Braunstein, S. R. King, J. Peti-Peterdi, K. Hanaoka, W. B. Guggino, L. M. Guay-Woodford, P. D. Bell, L. P. Sullivan, et al. Autocrine extracellular purinergic signaling in epithelial cells derived from polycystic kidneys Am J Physiol Renal Physiol, April 1, 2002; 282(4): F763 - F775. [Abstract] [Full Text] [PDF]