AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 267: F459-F466, 1994;
0363-6127/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chatzilias, A. A.
Right arrow Articles by Whiteside, C. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chatzilias, A. A.
Right arrow Articles by Whiteside, C. I.

AJP - Renal Physiology, Vol 267, Issue 3 459-F466, Copyright © 1994 by American Physiological Society

ARTICLES

Cellular mechanisms of glucose-induced myo-inositol transport upregulation in rat mesangial cells

A. A. Chatzilias and C. I. Whiteside
Department of Medicine, University of Toronto, Ontario, Canada.

Uptake of myo-inositol (MI) is necessary to maintain normal cellular phosphoinositide signaling and function. MI transport is up-regulated in the cells of diabetic rat glomeruli compared with normal rat glomeruli [C. I. Whiteside, J. C. Thompson, and J. Ohayon. Am. J. Physiol. 260 (Renal Fluid Electrolyte Physiol. 29): F138-F144, 1991]. To identify mechanisms associated with upregulation of MI transport, rat mesangial cells were cultured in high (25.6 mM) vs. normal (5.6 mM) glucose. Specific Na(+)-dependent [3H]MI uptake (> 97%), using L-[14C]glucose as the nonspecific marker, was linear for 120 min in high and normal glucose. In high glucose, compared with normal glucose, there was no change in Michaelis-Menten constant values [29.1 +/- 0.6 vs. 30.3 +/- 0.7 microM (SE)], whereas maximum velocity (Vmax) was increased (2.024 +/- 52 vs. 1.132 +/- 115 fmol.mg protein-1.min-1, P < 0.001). Mannitol (20.0 mM), used as an osmotic control, had no effect on the upregulation of MI transport. Maximum upregulation of MI transport measured by Vmax (control taken as 100%) was observed after 8 h of exposure to high glucose (222 +/- 6% above control, P < 0.0001) or galactose (20.0 mM) (194 +/- 6%, P < 0.0001) and was sustained for up to 48 h. The protein synthesis inhibitors cycloheximide (20 micrograms/ml) or actinomycin D (5 micrograms/ml), the F-actin depolymerizing agent cytochalasin D (2 micrograms/ml), and the aldose reductase inhibitor Tolrestat (0.3 mM) independently prevented glucose- or galactose-induced upregulation of MI transport.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
F. Porcellati, Y. Hosaka, T. Hlaing, M. Togawa, D. D. Larkin, A. Karihaloo, M. J. Stevens, P. D. Killen, and D. A. Greene
Alternate splicing in human Na+-MI cotransporter gene yields differentially regulated transport isoforms
Am J Physiol Cell Physiol, June 1, 1999; 276(6): C1325 - C1337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. Porcellati, T. Hlaing, M. Togawa, M. J. Stevens, D. D. Larkin, Y. Hosaka, T. W. Glover, D. N. Henry, D. A. Greene, and P. D. Killen
Human Na+-myo-inositol cotransporter gene: alternate splicing generates diverse transcripts
Am J Physiol Cell Physiol, May 1, 1998; 274(5): C1215 - C1225.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. A. Dlugosz, S. Munk, E. Ispanovic, H. J. Goldberg, and C. I. Whiteside
Mesangial cell filamentous actin disassembly and hypocontractility in high glucose are mediated by PKC-zeta
Am J Physiol Renal Physiol, January 1, 2002; 282(1): F151 - F163.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online