AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 267: F528-F536, 1994;
0363-6127/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grandaliano, G.
Right arrow Articles by Abboud, H. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grandaliano, G.
Right arrow Articles by Abboud, H. E.

AJP - Renal Physiology, Vol 267, Issue 4 528-F536, Copyright © 1994 by American Physiological Society

ARTICLES

Mitogenic signaling of thrombin in mesangial cells: role of tyrosine phosphorylation

G. Grandaliano, G. G. Choudhury, P. Biswas and H. E. Abboud
Department of Medicine, University of Texas Health Science Center at San Antonio.

Thrombin elicits multiple biological effects on a variety of cells. We have previously shown that thrombin is a potent mitogen for human glomerular mesangial cells. This mitogenic effect of thrombin is associated with activation of phospholipase C (PLC) and induction of platelet-derived growth factor (PDGF) gene expression. The thrombin receptor, which belongs to the guanine nucleotide binding protein (G protein)-coupled receptor family, has recently been shown to induce rapid tyrosine phosphorylation of cellular proteins. In the present study, we investigated the role of protein-tyrosine phosphorylation in mediating the cellular responses elicited by thrombin in human glomerular mesangial cells. Amino acid labeling followed by immunoprecipitation with phosphotyrosine antibodies demonstrate that thrombin stimulates tyrosine phosphorylation of a set of cellular proteins. Treatment of mesangial cells with thrombin followed by immunoblotting with phosphotyrosine antibodies showed three major bands of tyrosine-phosphorylated proteins approximately 130, 70, and 44-42 kDa. Phosphorylation of these proteins was inhibited by two tyrosine kinase inhibitors, herbimycin A and genistein. Both compounds inhibited DNA synthesis and PDGF B-chain gene expression but had no effect on inositol phosphates production or increases in cytosolic calcium in response to thrombin. These data demonstrate that protein-tyrosine phosphorylation is not required for thrombin-induced PLC activation with inositol phosphate formation and subsequent intracellular calcium release, but it is an absolute requirement for thrombin-induced DNA synthesis and PDGF B-chain gene expression.


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
R. MONNO, G. GRANDALIANO, R. FACCIO, E. RANIERI, C. MARTINO, L. GESUALDO, and F. P. SCHENA
Activated Coagulation Factor X: A Novel Mitogenic Stimulus for Human Mesangial Cells
J. Am. Soc. Nephrol., May 1, 2001; 12(5): 891 - 899.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
G. GRANDALIANO, R. MONNO, E. RANIERI, L. GESUALDO, F. P. SCHENA, C. MARTINO, and M. URSI
Regenerative and Proinflammatory Effects of Thrombin on Human Proximal Tubular Cells
J. Am. Soc. Nephrol., June 1, 2000; 11(6): 1016 - 1025.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Ghosh Choundhury, Y.-s. Kim, M. Simon, J. Wozney, S. Harris, N. Ghosh-Choundhury, and H. E. Abboud
Bone Morphogenetic Protein 2 Inhibits Platelet-derived Growth Factor-induced c-fos Gene Transcription and DNA Synthesis in Mesangial Cells. INVOLVEMENT OF MITOGEN-ACTIVATED PROTEIN KINASE
J. Biol. Chem., April 16, 1999; 274(16): 10897 - 10902.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online