AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 267: F654-F659, 1994;
0363-6127/94 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sagar, S.
Right arrow Articles by Singhal, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sagar, S.
Right arrow Articles by Singhal, P. C.

AJP - Renal Physiology, Vol 267, Issue 4 654-F659, Copyright © 1994 by American Physiological Society

ARTICLES

Morphine modulates 72-kDa matrix metalloproteinase

S. Sagar, D. Sorbi, L. A. Arbeit and P. C. Singhal
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park 11042.

Mesangial expansion is considered to be a precursor of glomerulosclerosis, a predominant glomerular lesion in heroin nephropathy. In addition to matrix synthesis, matrix degradation may also contribute to expansion of mesangium. In this study, we evaluated the effect of morphine on metalloproteinases (gelatinases) that degrade type IV collagen and are secreted by mesangial cells (MC). Gelatinolytic activity was significantly decreased in media of MC exposed to morphine for 1 wk compared with control [control, 2,411.6 +/- 198.7; morphine (10(-6) M), 954.4 +/- 112.2 ng.mg protein-1.3 h-1; P < 0.001]. A similar effect was seen at 2 wk [control, 17,010.6 +/- 1,789.5; morphine (10(-6) M), 8,925.2 +/- 1,623.5 ng.mg protein-1.3 h-1; P < 0.02]. Percent change in gelatinolytic activity was 39.58% (1 wk) and 47.53% (2 wk) compared with control. Morphine at concentrations of 10(-10) to 10(-6) M decreased gelatinolytic activity in MC. In in vivo studies, 24-h urines of morphine-treated rats showed a lower (P < 0.01) gelatinolytic activity when compared with controls. Isolated glomeruli from morphine-treated rats also showed decreased (P < 0.05) gelatinolytic activity compared with control. Naloxone, an opioid antagonist, did not inhibit the effect of morphine on gelatinolytic activity of MC. These results suggest that morphine may cause a decrease in degradation of type IV collagen in patients with heroin addiction. Accumulation of collagen because of lack of gelatinolytic activity in the mesangium may contribute to the expansion of mesangium.


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
O. LENZ, S. J. ELLIOT, and W. G. STETLER-STEVENSON
Matrix Metalloproteinases in Renal Development and Disease
J. Am. Soc. Nephrol., March 1, 2000; 11(3): 574 - 581.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online