| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 270, Issue 1 106-F115, Copyright © 1996 by American Physiological Society
ARTICLES |
A. Roczniak and K. D. Burns
Department of Medicine, University of Ottawa, Ontario, Canada.
The proximal tubule contains the target for nitric oxide (NO), soluble guanylate cyclase, and has the capacity for NO production. Inhibition of renal NO synthesis reduces fractional excretion of lithium, suggesting an inhibitory effect of NO on proximal tubule Na+ transport. The present studies determined direct effects of donors of NO in rabbit proximal tubule. In both freshly isolated proximal tubule segments and in primary cultures of proximal tubule cells, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP) caused dose-dependent increases in guanosine 3',5'-cyclic monophosphate (cGMP). SNAP was more potent than SNP in stimulating cGMP; this was associated with an enhanced production of nitrite, the stable end-product of NO. In rabbit proximal tubule cells, SNP or SNAP (10(-3) M) significantly inhibited the activity of the apical Na+/H+ exchanger, determined by assay of amiloride-sensitive 22Na+ uptake (% inhibition: SNP, 34.90 +/- 5.52%, P < 0.001; SNAP, 30.77 +/- 8.20%, P < 0.002). To determine the role of cGMP in mediating these effects, proximal tubule cells were incubated with the membrane-permeable analogue, 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP). Na+/H+ exchange was significantly inhibited by 8-BrcGMP (10(3)M) (% inhibition: 32.40 +/- 9.06%: P < 0.05). The inhibitor of soluble guanylate cyclase, LY-83583, caused partial inhibition of SNP-stimulated cGMP generation and partly blocked the inhibitory effect of SNP on Na+/H+ exchange. Protein kinase A (PKA) activity was not stimulated by SNP, indicating that potential cross-activation of PKA by cGMP did not mediate the effects of NO donors. These data indicate that NO stimulates soluble guanylate cyclase in rabbit proximal tubule and causes inhibition of Na-/H+ exchange. This is at least partly mediated by generation of cGMP. We conclude that NO is an important autocrine or paracrine factor directly regulating Na+ transport in the proximal tubule.
This article has been cited by other articles:
|
|
 |
|
  F. Ahmed, B. A. Kemp, N. L. Howell, H. M. Siragy, and R. M. Carey Extracellular Renal Guanosine Cyclic 3'5'-Monophosphate Modulates Nitric Oxide and Pressure-Induced Natriuresis Hypertension, November 1, 2007; 50(5): 958 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Hakam and T. Hussain Angiotensin II AT2 receptors inhibit proximal tubular Na+-K+-ATPase activity via a NO/cGMP-dependent pathway Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1430 - F1436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nakamura, J. Hirano, and M. Kubokawa Regulation of an inwardly rectifying K+ channel by nitric oxide in cultured human proximal tubule cells Am J Physiol Renal Physiol, September 1, 2004; 287(3): F411 - F417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Massaro, L. W. Lee, A. B. Patel, D. S. Wu, M.-J. Yu, B. N. Scott, D. A. Schooley, K. M. Schegg, and K. W. Beyenbach The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules of Aedes aegypti J. Exp. Biol., July 15, 2004; 207(16): 2877 - 2888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Notenboom, D. S. Miller, P. Smits, F. G. M. Russel, and R. Masereeuw Involvement of guanylyl cyclase and cGMP in the regulation of Mrp2-mediated transport in the proximal tubule Am J Physiol Renal Physiol, July 1, 2004; 287(1): F33 - F38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Graebe, L. Brond, S. Christensen, S. Nielsen, N. V. Olsen, and T. E. N. Jonassen Chronic nitric oxide synthase inhibition exacerbates renal dysfunction in cirrhotic rats Am J Physiol Renal Physiol, February 1, 2004; 286(2): F288 - F297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zimpelmann, N. Li, and K. D. Burns Nitric oxide inhibits superoxide-stimulated urea permeability in the rat inner medullary collecting duct Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1160 - F1167. [Abstract] [Full Text]
|
|
|
|
|
|
H. He, T. Podymow, J. Zimpelmann, and K. D. Burns NO inhibits Na+-K+-2Cl- cotransport via a cytochrome P-450-dependent pathway in renal epithelial cells (MMDD1) Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1235 - F1244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Ahmed, N. Ashton, and R. J. Balment The Effect of Chloroquine on Renal Function and Vasopressin Secretion: A Nitric Oxide-Dependent Effect J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 156 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Saksena, R. K. Gill, I. A. Syed, S. Tyagi, W. A. Alrefai, K. Ramaswamy, and P. K. Dudeja Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxide Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G626 - G633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Gill, S. Saksena, I. A. Syed, S. Tyagi, W. A. Alrefai, J. Malakooti, K. Ramaswamy, and P. K. Dudeja Regulation of NHE3 by nitric oxide in Caco-2 cells Am J Physiol Gastrointest Liver Physiol, September 1, 2002; 283(3): G747 - G756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baines and P. Ho Glucose stimulates O2 consumption, NOS, and Na/H exchange in diabetic rat proximal tubules Am J Physiol Renal Physiol, August 1, 2002; 283(2): F286 - F293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Ortiz and J. L. Garvin Role of nitric oxide in the regulation of nephron transport Am J Physiol Renal Physiol, May 1, 2002; 282(5): F777 - F784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Pagel, T. Langenickel, K. Hohnel, S. Philipp, A. K. Nussler, W. F. Blum, M. L. Aubert, R. Dietz, and R. Willenbrock Cardiac and Renal Effects of Growth Hormone in Volume Overload-Induced Heart Failure: Role of NO Hypertension, January 1, 2002; 39(1): 57 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang, T. J. Berndt, and F. G. Knox Mechanism underlying diuretic effect of L-NAME at a subpressor dose Am J Physiol Renal Physiol, September 1, 2001; 281(3): F414 - F419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-H. Jin, H. M. Siragy, and R. M. Carey Renal Interstitial cGMP Mediates Natriuresis by Direct Tubule Mechanism Hypertension, September 1, 2001; 38(3): 309 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zimpelmann and K. D. Burns Angiotensin II AT2 receptors inhibit growth responses in proximal tubule cells Am J Physiol Renal Physiol, August 1, 2001; 281(2): F300 - F308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Diaz-Sylvester, M. Mac Laughlin, and C. Amorena Peritubular fluid viscosity modulates H+ flux in proximal tubules through NO release Am J Physiol Renal Physiol, February 1, 2001; 280(2): F239 - F243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Ortiz and J. L. Garvin NO Inhibits NaCl Absorption by Rat Thick Ascending Limb Through Activation of cGMP-Stimulated Phosphodiesterase Hypertension, February 1, 2001; 37(2): 467 - 471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang, F. M. Inglis, and R. G. Kalb Defective fluid and HCO3- absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice Am J Physiol Renal Physiol, September 1, 2000; 279(3): F518 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. D. Vaziri, Y. Ding, D. S. Sangha, and R. E. Purdy Upregulation of NOS by simulated microgravity, potential cause of orthostatic intolerance J Appl Physiol, July 1, 2000; 89(1): 338 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Liang and F. G. Knox Production and functional roles of nitric oxide in the proximal tubule Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2000; 278(5): R1117 - R1124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Plato, E. G. Shesely, and J. L. Garvin eNOS Mediates L-Arginine-Induced Inhibition of Thick Ascending Limb Chloride Flux Hypertension, January 1, 2000; 35(1): 319 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liang and F. G. Knox Nitric oxide activates PKCalpha and inhibits Na+-K+-ATPase in opossum kidney cells Am J Physiol Renal Physiol, December 1, 1999; 277(6): F859 - F865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Turkstra, P. Boer, B. Braam, and H. A. Koomans Increased Availability of Nitric Oxide Leads to Enhanced Nitric Oxide Dependency of Tubuloglomerular Feedback in the Contralateral Kidney of Rats With 2-Kidney, 1-Clip Goldblatt Hypertension Hypertension, October 1, 1999; 34(4): 679 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Garvin and N. J. Hong Nitric oxide inhibits sodium/hydrogen exchange activity in the thick ascending limb Am J Physiol Renal Physiol, September 1, 1999; 277(3): F377 - F382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Kone and S. Higham Nitric oxide inhibits transcription of the Na+-K+-ATPase alpha 1-subunit gene in an MTAL cell line Am J Physiol Renal Physiol, April 1, 1999; 276(4): F614 - F621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Elmer, K. G. Brady, M. L. Drumm, and T. J. Kelley Nitric oxide-mediated regulation of transepithelial sodium and chloride transport in murine nasal epithelium Am J Physiol Lung Cell Mol Physiol, March 1, 1999; 276(3): L466 - L473. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Plato, B. A. Stoos, D. Wang, and J. L. Garvin Endogenous nitric oxide inhibits chloride transport in the thick ascending limb Am J Physiol Renal Physiol, January 1, 1999; 276(1): F159 - F163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Broere, A. H. Van Den Meiracker, F. Boomsma, F. H. M. Derkx, A. J. Man In'T Veld, and M. A. D. H. Schalekamp Human renal and systemic hemodynamic, natriuretic, and neurohumoral responses to different doses of L-NAME Am J Physiol Renal Physiol, December 1, 1998; 275(6): F870 - F877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Azam, G. Gupta, W. Chen, S. Wellington, D. Warburton, and R. S. Danziger Genetic Mapping of Soluble Guanylyl Cyclase Genes : Implications for Linkage to Blood Pressure in the Dahl Rat Hypertension, July 1, 1998; 32(1): 149 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Laycock, T. Vogel, P. R. Forfia, J. Tuzman, X. Xu, M. Ochoa, C. I. Thompson, A. Nasjletti, and T. H. Hintze Role of Nitric Oxide in the Control of Renal Oxygen Consumption and the Regulation of Chemical Work in the Kidney Circ. Res., June 29, 1998; 82(12): 1263 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Bech, C. B. Nielsen, P. Ivarsen, K. T. Jensen, and E. B. Pedersen Dietary sodium affects systemic and renal hemodynamic response to NO inhibition in healthy humans Am J Physiol Renal Physiol, May 1, 1998; 274(5): F914 - F923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lu, X. Wang, and W. Wang Nitric oxide increases the activity of the apical 70-pS K+ channel in TAL of rat kidney Am J Physiol Renal Physiol, May 1, 1998; 274(5): F946 - F950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Eitle, S. Hiranyachattada, H. Wang, and P. J. Harris Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney Am J Physiol Cell Physiol, April 1, 1998; 274(4): C1075 - C1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Escalante, J. C. McGiff, and A. O. Oyekan Role of cytochrome P-450 arachidonate metabolites in endothelin signaling in rat proximal tubule Am J Physiol Renal Physiol, January 1, 2002; 282(1): F144 - F150. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
