AJP - Renal Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 270: F154-F163, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Silbernagl, S.
Right arrow Articles by Dantzler, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Silbernagl, S.
Right arrow Articles by Dantzler, W. H.

AJP - Renal Physiology, Vol 270, Issue 1 154-F163, Copyright © 1996 by American Physiological Society

ARTICLES

Compartmentation of amino acids in the rat kidney

S. Silbernagl, K. Volker and W. H. Dantzler
Physiologisches Institut, Universitat Wurzburg, Germany.

Amino acid concentrations ([AA]) were determined in cortical, outer and inner medullary (OM and IM), and papillary tissue of rat kidney (Cti, mmol/kg wet wt), in plasma (Cpl), and in urine. In all regions, Cti values were highest for Tau, Gly, and Glu-, making up 54-65% of the total [AA]:27, 21, and 11 mmol/kg wet wt in cortex, OM, and IM and papilla, respectively. Cortical cell water [AA] values (CcH2O, mmol/kgH2O) were between 12.4 (Tau) and 0.09 (Orn+), representing cell water-to-plasma water ratios (CcH2O/CpH2O) between 134 (Asp-) and 0.9 (Thr and Cit). Short-term water diuresis did not change the total tissue [AA] throughout the kidney. Treatment of the tissue with Triton X-100 instead of sulfosalicylic acid (SSA) resulted in much higher [AA], except for Glu-, Glu-NH2, Tau, and exogenous L-homoarginine+ (hoArg+). When hoArg+ was infused (leading to a Cpl = 5.9 mmol/l), Cti of hoArg+ was similar throughout the kidney (13-22 mmol/kg wet wt). In the presence of hoArg+, CcH2O/CpH2O of Arg+ rose 13-fold. We conclude that 1) AA contribute 20% to cytosolic osmolality in renal cortex, 2) total [AA] decreases from cortex to papilla, 3) cellular uptake of Tau and anionic AA must be rheogenic, whereas cationic AA (except for Arg+ in cortex) are passively distributed, and 4) AA do not seem to contribute quantitatively to short-term medullary osmotic adaptation during diuresis.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Wu, B. Cholewa, and D. L. Mattson
Characterization of L-arginine transporters in rat renal inner medullary collecting duct
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2000; 278(6): R1506 - R1512.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J.-F. Noulin, E. Brochiero, J.-Y. Lapointe, and R. Laprade
Two types of K+ channels at the basolateral membrane of proximal tubule: inhibitory effect of taurine
Am J Physiol Renal Physiol, August 1, 1999; 277(2): F290 - F297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Silbernagl, K. Volker, and W. H. Dantzler
D-Serine is reabsorbed in rat renal pars recta
Am J Physiol Renal Physiol, June 1, 1999; 276(6): F857 - F863.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online