AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 270: F657-F668, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frokiaer, J.
Right arrow Articles by Nielsen, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frokiaer, J.
Right arrow Articles by Nielsen, S.

AJP - Renal Physiology, Vol 270, Issue 4 657-F668, Copyright © 1996 by American Physiological Society

ARTICLES

Bilateral ureteral obstruction downregulates expression of vasopressin-sensitive AQP-2 water channel in rat kidney

J. Frokiaer, D. Marples, M. A. Knepper and S. Nielsen
Department of Clinical Physiology, Aarhus University Hospital, Denmark.

Polyuria after release of bilateral ureteral obstruction (BUO) is frequently seen in patients with urological disorders. In this study, we examined the effect of BUO and release of BUO on the expression of the vasopressin-regulated water channel aquaporin-2 (AQP-2) in rat kidney. Ureters were obstructed for 24 h in all experiments, and BUO was either not released or released for 24 or 48 h or 7 days. Each group of experimental rats were matched with sham-operated controls. One kidney was used for membrane fractionation and immunoblotting, whereas the contralateral was fixed for immunocytochemistry. Immunoblotting demonstrated a significant reduction in AQP-2 expression in inner medullar during 24 h of BUO to 26 +/- 8% (P < 0.001). Release of BUO was associated with immediate onset of a predominant osmotic-dependent polyuria. Forty-eight hours after release of BUO, the reduction in AQP-2 expression persisted (19 +/- 8%, P < 0.001), concurrent with a marked nonosmotic postobstructive polyuria, as determined by a significant reduction in free-water clearance (-50 +/- 7 vs. -85 +/- 10 microliters.min-1.kg-1, P < 0.05). Immunofluorescence and immunoelectron microscopy confirmed the reduced levels of AQP-2 in collecting duct principal cells. Seven days after release, the renal excretion of water and electrolytes had almost normalized. However, the downregulation of AQP-2 was not partly reversed (49 +/- 14%, P < 0.001), and, consistent with this, the urinary concentrating capacity was significantly reduced 7 days after release to a 18-h period of thirst. This strongly suggests that the persistent downregulation of AQP-2 is the cause of the slow recovery in concentration capacity. In conclusion, BUO and release of BUO were associated with a marked reduction in expression of AQP-2, coincident with the development and maintenance of postobstructive polyuria. Thus reduced AQP-2 levels may represent an important factor in the slow recovery from postobstructive diuresis.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Jensen, E. H. Bae, R. A. Fenton, R. Norregaard, S. Nielsen, S. W. Kim, and J. Frokiaer
Angiotensin II regulates V2 receptor and pAQP2 during ureteral obstruction
Am J Physiol Renal Physiol, January 1, 2009; 296(1): F127 - F134.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Wang, C. Li, S. W. Kim, T. Ring, J. Wen, J. C. Djurhuus, W. Wang, S. Nielsen, and J. Frokiaer
Ureter obstruction alters expression of renal acid-base transport proteins in rat kidney
Am J Physiol Renal Physiol, August 1, 2008; 295(2): F497 - F506.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. A. Fenton, L. Brond, S. Nielsen, and J. Praetorius
Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F748 - F760.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, W. Wang, R. Norregaard, M. A. Knepper, S. Nielsen, and J. Frokiaer
Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F333 - F341.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Norregaard, B. L. Jensen, S. O. Topcu, M. Diget, H. Schweer, M. A. Knepper, S. Nielsen, and J. Frokiaer
COX-2 activity transiently contributes to increased water and NaCl excretion in the polyuric phase after release of ureteral obstruction
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1322 - F1333.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Eskild-Jensen, K. Thomsen, C. Rungo, L. S. Ferreira, L. F. Paulsen, Y. F. Rawashdeh, J. R. Nyengaard, S. Nielsen, J. C. Djurhuus, and J. Frokiaer
Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F921 - F929.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. M. Jensen, C. Li, H. A. Praetorius, R. Norregaard, S. Frische, M. A. Knepper, S. Nielsen, and J. Frokiaer
Angiotensin II mediates downregulation of aquaporin water channels and key renal sodium transporters in response to urinary tract obstruction
Am J Physiol Renal Physiol, November 1, 2006; 291(5): F1021 - F1032.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. H. Robben, N. V. A. M. Knoers, and P. M. T. Deen
Cell biological aspects of the vasopressin type-2 receptor and aquaporin 2 water channel in nephrogenic diabetes insipidus.
Am J Physiol Renal Physiol, August 1, 2006; 291(2): F257 - F270.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, Y. Shi, W. Wang, C. Sardeli, T.-H. Kwon, K. Thomsen, T. Jonassen, J. C. Djurhuus, M. A. Knepper, S. Nielsen, et al.
{alpha}-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction
Am J Physiol Renal Physiol, February 1, 2006; 290(2): F384 - F396.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Norregaard, B. L. Jensen, C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiaer
COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F322 - F333.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, J. D. Klein, W. Wang, M. A. Knepper, S. Nielsen, J. M. Sands, and J. Frokiaer
Altered expression of urea transporters in response to ureteral obstruction
Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1154 - F1162.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiaer
Altered expression of major renal Na transporters in rats with bilateral ureteral obstruction and release of obstruction
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F889 - F901.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiar
Downregulation of renal aquaporins in response to unilateral ureteral obstruction
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1066 - F1079.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiar
Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F155 - F166.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper
Aquaporins in the Kidney: From Molecules to Medicine
Physiol Rev, January 1, 2002; 82(1): 205 - 244.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. W. KIM, S. H. CHO, B. S. OH, C. H. YEUM, K. C. CHOI, K. Y. AHN, and J. LEE
Diminished Renal Expression of Aquaporin Water Channels in Rats with Experimental Bilateral Ureteral Obstruction
J. Am. Soc. Nephrol., October 1, 2001; 12(10): 2019 - 2028.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Li, W. Wang, T.-H. Kwon, L. Isikay, J. G. Wen, D. Marples, J. C. Djurhuus, A. Stockwell, M. A. Knepper, S. Nielsen, et al.
Downregulation of AQP1, -2, and -3 after ureteral obstruction is associated with a long-term urine-concentrating defect
Am J Physiol Renal Physiol, July 1, 2001; 281(1): F163 - F171.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S.-W. KIM, J.-U. LEE, M.-Y. NAH, D.-G. KANG, K.-Y. AHN, H.-S. LEE, and K.-C. CHOI
Cisplatin Decreases the Abundance of Aquaporin Water Channels in Rat Kidney
J. Am. Soc. Nephrol., May 1, 2001; 12(5): 875 - 882.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. E. N. Jonassen, S. Christensen, T.-H. Kwon, S. Langhoff, N. Salling, and S. Nielsen
Renal water handling in rats with decompensated liver cirrhosis
Am J Physiol Renal Physiol, December 1, 2000; 279(6): F1101 - F1109.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. VALENTI, A. LAERA, G. PACE, G. ACETO, M. L. LOSPALLUTI, R. PENZA, F. P. SELVAGGI, M. L. CHIOZZA, and M. SVELTO
Urinary Aquaporin 2 and Calciuria Correlate with the Severity of Enuresis in Children
J. Am. Soc. Nephrol., October 1, 2000; 11(10): 1873 - 1881.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
P. AGRE
Aquaporin Water Channels in Kidney
J. Am. Soc. Nephrol., April 1, 2000; 11(4): 764 - 777.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. E. N. Jonassen, D. Promeneur, S. Christensen, J. S. Petersen, and S. Nielsen
Decreased vasopressin-mediated renal water reabsorption in rats with chronic aldosterone-receptor blockade
Am J Physiol Renal Physiol, February 1, 2000; 278(2): F246 - F256.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Junaid, L. Cui, S. B. Penner, and D. D. Smyth
Regulation of Aquaporin-2 Expression by the alpha 2-Adrenoceptor Agonist Clonidine in the Rat
J. Pharmacol. Exp. Ther., November 1, 1999; 291(2): 920 - 923.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
P. FERNÁNDEZ-LLAMA, R. TURNER, G. DIBONA, and M. A. KNEPPER
Renal Expression of Aquaporins in Liver Cirrhosis Induced by Chronic Common Bile Duct Ligation in Rats
J. Am. Soc. Nephrol., September 1, 1999; 10(9): 1950 - 1957.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T.-H. Kwon, J. Frokiaer, P. Fernandez-Llama, M. A. Knepper, and S. Nielsen
Reduced abundance of aquaporins in rats with bilateral ischemia-induced acute renal failure: prevention by alpha -MSH
Am J Physiol Renal Physiol, September 1, 1999; 277(3): F413 - F427.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
P. FERNÁNDEZ-LLAMA, P. ANDREWS, R. TURNER, S. SAGGI, J. DIMARI, T.-H. KWON, S. NIELSEN, R. SAFIRSTEIN, and M. A. KNEPPER
Decreased Abundance of Collecting Duct Aquaporins in Post-Ischemic Renal Failure in Rats
J. Am. Soc. Nephrol., August 1, 1999; 10(8): 1658 - 1668.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
H. WEN, J. FRØKI&Aelig;R, T.-H. KWON, and S. NIELSEN
Urinary Excretion of Aquaporin-2 in Rat Is Mediated by a Vasopressin-Dependent Apical Pathway
J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1416 - 1429.
[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
S. NIELSEN, T.-H. KWON, B. M. CHRISTENSEN, D. PROMENEUR, J. FRØKI&Aelig;R, and D. MARPLES
Physiology and Pathophysiology of Renal Aquaporins
J. Am. Soc. Nephrol., March 1, 1999; 10(3): 647 - 663.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Marples, J. Frokiaer, and S. Nielsen
Long-term regulation of aquaporins in the kidney
Am J Physiol Renal Physiol, March 1, 1999; 276(3): F331 - F339.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Frokiaer, D. Marples, H. Valtin, J. F. Morris, M. A. Knepper, and S. Nielsen
Low aquaporin-2 levels in polyuric DI +/+ severe mice with constitutively high cAMP-phosphodiesterase activity
Am J Physiol Renal Physiol, February 1, 1999; 276(2): F179 - F190.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen
Reduced AQP1, -2, and -3 levels in kidneys of rats with CRF induced by surgical reduction in renal mass
Am J Physiol Renal Physiol, November 1, 1998; 275(5): F724 - F741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. E. N. Jonassen, S. Nielsen, S. Christensen, and J. S. Petersen
Decreased vasopressin-mediated renal water reabsorption in rats with compensated liver cirrhosis
Am J Physiol Renal Physiol, August 1, 1998; 275(2): F216 - F225.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. M. Christensen, D. Marples, U. B. Jensen, J. Frokiaer, D. Sheikh-Hamad, M. Knepper, and S. Nielsen
Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution
Am J Physiol Renal Physiol, August 1, 1998; 275(2): F285 - F297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. M. Sands, F. X. Flores, A. Kato, M. A. Baum, E. M. Brown, D. T. Ward, S. C. Hebert, and H. W. Harris
Vasopressin-elicited water and urea permeabilities are altered in IMCD in hypercalcemic rats
Am J Physiol Renal Physiol, May 1, 1998; 274(5): F978 - F985.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Nielsen, J. Terris, D. Andersen, C. Ecelbarger, J. Frokiar, T. Jonassen, D. Marples, M. A. Knepper, and J. S. Petersen
Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct
PNAS, May 13, 1997; 94(10): 5450 - 5455.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online