AJP - Renal Physiology, Vol 270, Issue 5 766-F775, Copyright © 1996 by American Physiological Society

ARTICLES

Differential expression of PKC isoforms in fresh and cultured rabbit CCD

T. W. Wilborn and J. A. Schafer
Department of Physiology and Biophysics, University of Alabama at Birmingham 35294, USA.

Growth of rabbit cortical collecting duct (CCD) cells in primary culture results in a phenotype in which there is a stable stimulation of Na+ transport by arginine vasopressin. Our objective was to determine whether this altered phenotype was associated with altered expression of protein kinase C (PKC) isoforms. Western blot analysis of extracted proteins and reverse transcription-polymerase chain reaction analysis of extracted RNA showed expression of PKC-alpha, -epsilon, and -zeta in microdissected CCD segments and in fresh and cultured immunodissected CCD cells. These techniques also suggested that the rabbit CCD expresses PKC-eta- and -theta-like isoforms, which have not been identified in this species. Growth of CCD cells in primary culture produced no apparent change in the level of expression of PKC-alpha, -epsilon, or -zeta; however, the theta-like isoform was strongly expressed in fresh CCDs but only weakly expressed in cultured CCDs. The putative eta-isoform was more heavily expressed in cultured than in fresh CCD cells. The consequences of altered expression of these two isoforms in fresh and cultured rabbit CCD remain unknown, but the possibility exists that they are involved in the altered arginine vasopressin response of cultured cells.

This article has been cited by other articles:

				S. Redling, I. L. Pfaff, M. Leitges, and V. Vallon Immunolocalization of protein kinase C isoenzymes {alpha}, {beta}I, {beta}II, {delta}, and {varepsilon} in mouse kidney Am J Physiol Renal Physiol, August 1, 2004; 287(2): F289 - F298. [Abstract] [Full Text] [PDF]

				H. Sterling, D.-H. Lin, Y.-J. Chen, Y. Wei, Z.-J. Wang, J. Lai, and W.-H. Wang PKC expression is regulated by dietary K intake and mediates internalization of SK channels in the CCD Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1072 - F1078. [Abstract] [Full Text] [PDF]

				M. S. Awayda, J. D. Platzer, R. L. Reger, and A. Bengrine Role of PKCalpha in feedback regulation of Na+ transport in an electrically tight epithelium Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1122 - C1132. [Abstract] [Full Text] [PDF]

				J. A. Schafer Abnormal regulation of ENaC: syndromes of salt retention and salt wasting by the collecting duct Am J Physiol Renal Physiol, August 1, 2002; 283(2): F221 - F235. [Abstract] [Full Text] [PDF]

				D. Sun, T. W. Wilborn, and J. A. Schafer Dopamine D4 receptor isoform mRNA and protein are expressed in the rat cortical collecting duct Am J Physiol Renal Physiol, November 1, 1998; 275(5): F742 - F751. [Abstract] [Full Text] [PDF]

				T. W. Wilborn, D. Sun, and J. A. Schafer Expression of multiple alpha -adrenoceptor isoforms in rat CCD Am J Physiol Renal Physiol, July 1, 1998; 275(1): F111 - F118. [Abstract] [Full Text] [PDF]

				N. Meller, A. Altman, and N. Isakov New Perspectives on PKC{theta}, a Member of the Novel Subfamily of Protein Kinase C Stem Cells, May 1, 1998; 16(3): 178 - 192. [Abstract] [Full Text]

				M. A. Lal, P. R. Proulx, and R. L. Hebert A role for PKCepsilon and MAP kinase in bradykinin-induced arachidonic acid release in rabbit CCD cells Am J Physiol Renal Physiol, April 1, 1998; 274(4): F728 - F735. [Abstract] [Full Text] [PDF]

				C.-L. Chou, S. I. Rapko, and M. A. Knepper Phosphoinositide signaling in rat inner medullary collecting duct Am J Physiol Renal Physiol, March 1, 1998; 274(3): F564 - F572. [Abstract] [Full Text] [PDF]

				J. A. Schafer, M. L. Watkins, L. Li, P. Herter, S. Haxelmans, and E. Schlatter A simplified method for isolation of large numbers of defined nephron segments Am J Physiol Renal Physiol, October 1, 1997; 273(4): F650 - F657. [Abstract] [Full Text] [PDF]