AJP - Renal Physiology, Vol 270, Issue 5 886-F899, Copyright © 1996 by American Physiological Society

ARTICLES

Endogenous origin of glomerular endothelial and mesangial cells in grafts of embryonic kidneys

D. P. Hyink, D. C. Tucker, P. L. St John, V. Leardkamolkarn, M. A. Accavitti, C. K. Abrass and D. R. Abrahamson
Department of Cell Biology, University of Alabama at Birmingham 35294, USA.

To address origins of glomerular endothelial and mesangial cells in embryonic mammalian kidneys, we established interspecies grafts between rats and mice, in which fetal kidneys were implanted into the anterior eye chamber of adult hosts. After 5-7 days, hosts bearing grafts received intravenous injections with species-specific monoclonal antibodies (MAbs) to matrix components. In all cases, glomerular basement membranes and mesangial matrices labeled solely for donor-derived matrix. Additionally, microvessel extracellular matrices within grafts were usually of donor origin. To examine directly the origin of glomerular endothelial and mesangial cells, we grafted embryonic gestational days 11-12 (E11-12) kidneys from normal mice into anterior eye chambers of host reverse-orientation splice acceptor 26 mice, which are transgenic animals that express beta-galactosidase in every cell. When grafts were developed for beta-galactosidase activity, host cells were seen in peripheral vessels, but the majority of glomerular endothelial cells were of donor, not host, origin. Where host-derived-endothelial cells were found in glomeruli, donor endothelial cells were present as well. Mesangial cells were always of donor origin. When E11 mouse kidneys were labeled with the endothelial cell-specific Bandeiraea simplicifolia isolectin B4, we determined that endothelial cells are present from the inception of metanephrogenesis. Together, the evidence shows that cells of endogenous kidney origin were almost entirely responsible for development of the glomerular microvasculature in oculo. External vessels from the host, although important for graft maintenance, were not major contributors to the glomerulus.

This article has been cited by other articles:

				M. J. Mondrinos, S. Koutzaki, P. I. Lelkes, and C. M. Finck A tissue-engineered model of fetal distal lung tissue Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L639 - L650. [Abstract] [Full Text] [PDF]

				T. Akimoto and M. R. Hammerman Microvessel formation from mouse aorta is stimulated in vitro by secreted VEGF and extracts from metanephroi Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1625 - C1632. [Abstract] [Full Text] [PDF]

				J. M. Ricono, Y.-C. Xu, M. Arar, D.-c. Jin, J. L. Barnes, and H. E. Abboud Morphological Insights into the Origin of Glomerular Endothelial and Mesangial Cells and Their Precursors J. Histochem. Cytochem., February 1, 2003; 51(2): 141 - 150. [Abstract] [Full Text] [PDF]

				M. R. Hammerman Xenotransplantation of developing kidneys Am J Physiol Renal Physiol, October 1, 2002; 283(4): F601 - F606. [Abstract] [Full Text] [PDF]

				B. Dekel, N. Amariglio, N. Kaminski, A. Schwartz, E. Goshen, F. D. Arditti, I. Tsarfaty, J. H. Passwell, Y. Reisner, and G. Rechavi Engraftment and Differentiation of Human Metanephroi into Functional Mature Nephrons after Transplantation into Mice Is Accompanied by a Profile of Gene Expression Similar to Normal Human Kidney Development J. Am. Soc. Nephrol., April 1, 2002; 13(4): 977 - 990. [Abstract] [Full Text] [PDF]

				T. Ito, A. Suzuki, E. Imai, M. Okabe, and M. Hori Bone Marrow Is a Reservoir of Repopulating Mesangial Cells during Glomerular Remodeling J. Am. Soc. Nephrol., December 1, 2001; 12(12): 2625 - 2635. [Abstract] [Full Text] [PDF]

				S. A. Rogers and M. R. Hammerman Transplantation of metanephroi after preservation in vitro Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2001; 281(2): R661 - R665. [Abstract] [Full Text] [PDF]

				M. L. S. Sequeira Lopez, E. S. Pentz, B. Robert, D. R. Abrahamson, and R. A. Gomez Embryonic origin and lineage of juxtaglomerular cells Am J Physiol Renal Physiol, August 1, 2001; 281(2): F345 - F356. [Abstract] [Full Text] [PDF]

				S. A. Rogers and M. R. Hammerman Transplantation of rat metanephroi into mice Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1865 - R1869. [Abstract] [Full Text] [PDF]

				S. A. Rogers, H. Liapis, and M. R. Hammerman Transplantation of metanephroi across the major histocompatibility complex in rats Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2001; 280(1): R132 - R136. [Abstract] [Full Text] [PDF]

				H. T. YUAN, C. SURI, D. N. LANDON, G. D. YANCOPOULOS, and A. S. WOOLF Angiopoietin-2 Is a Site-Specific Factor in Differentiation of Mouse Renal Vasculature J. Am. Soc. Nephrol., June 1, 2000; 11(6): 1055 - 1066. [Abstract] [Full Text]

				A. TUFRO, V. F. NORWOOD, R. M. CAREY, and R. A. GOMEZ Vascular Endothelial Growth Factor Induces Nephrogenesis and Vasculogenesis J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2125 - 2134. [Abstract] [Full Text]

				T. TAKAHASHI, K. TAKAHASHI, R. MERNAUGH, V. DROZDOFF, C. SIPE, H. SCHOECKLMANN, B. ROBERT, D. R. ABRAHAMSON, and T. O. DANIEL Endothelial Localization of Receptor Tyrosine Phosphatase, ECRTP/DEP-1, in Developing and Mature Renal Vasculature J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2135 - 2145. [Abstract] [Full Text]

				A. T. Clark and J. F. Bertram Molecular regulation of nephron endowment Am J Physiol Renal Physiol, April 1, 1999; 276(4): F485 - F497. [Abstract] [Full Text] [PDF]

				B. Robert, P. L. St. John, and D. R. Abrahamson Direct visualization of renal vascular morphogenesis in Flk1 heterozygous mutant mice Am J Physiol Renal Physiol, July 1, 1998; 275(1): F164 - F172. [Abstract] [Full Text] [PDF]

				L. M. Antes, M. M. Villar, S. Decker, R. F. Nicosia, and D. A. Kujubu A serum-free in vitro model of renal microvessel development Am J Physiol Renal Physiol, June 1, 1998; 274(6): F1150 - F1160. [Abstract] [Full Text] [PDF]

				J. Barasch, J. Qiao, G. McWilliams, D. Chen, J. A. Oliver, and D. Herzlinger Ureteric bud cells secrete multiple factors, including bFGF, which rescue renal progenitors from apoptosis Am J Physiol Renal Physiol, November 1, 1997; 273(5): F757 - F767. [Abstract] [Full Text] [PDF]

				J. M. Ricono, M. Arar, G. G. Choudhury, and H. E. Abboud Effect of platelet-derived growth factor isoforms in rat metanephric mesenchymal cells Am J Physiol Renal Physiol, February 1, 2002; 282(2): F211 - F219. [Abstract] [Full Text] [PDF]