AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 270: F1038-F1048, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morales, M. M.
Right arrow Articles by Guggino, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morales, M. M.
Right arrow Articles by Guggino, W. B.

AJP - Renal Physiology, Vol 270, Issue 6 1038-F1048, Copyright © 1996 by American Physiological Society

ARTICLES

Both the wild type and a functional isoform of CFTR are expressed in kidney

M. M. Morales, T. P. Carroll, T. Morita, E. M. Schwiebert, O. Devuyst, P. D. Wilson, A. G. Lopes, B. A. Stanton, H. C. Dietz, G. R. Cutting and W. B. Guggino
Department of Physiology, Johns Hopkins University School of Medicine Baltimore, Maryland 21205, USA.

The cystic fibrosis transmembrane conductance regulator (CFTR) consists of five domains, two transmembrane-spanning domains, each composed of six transmembrane segments, a regulatory domain, and two nucleotide-binding domains (NBDs). CFTR is expressed in kidney, but its role in overall renal function is not well understood, because mutations in CFTR found in patients with cystic fibrosis are not associated with renal dysfunction. To learn more about the distribution and functional forms of CFTR in kidney, we used a combination of molecular, cell biological, and electrophysiological approaches. These include an evaluation of CFTR mRNA and protein expression, as well as both two-electrode and patch clamping of CFTR expressed either in Xenopus oocytes or mammalian cells. In addition to wild-type CFTR mRNA, an alternate form containing only the first transmembrane domain (TMD), the first NBD, and the regulatory domain (TNR-CFTR) is expressed in kidney. Although missing the second set of TMDs and the second NBD, when expressed in Xenopus oocytes, TNR-CFTR has cAMP-dependent protein kinase A (PKA)-stimulated single Cl- channel characteristics and regulation of PKA activation of outwardly rectifying Cl- channels that are very similar to those of wild-type CFTR. TNR-CFTR mRNA is produced by an unusual mRNA processing mechanism and is expressed in a tissue-specific manner primarily in renal medulla.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
S. C. Hebert, G. Desir, G. Giebisch, and W. Wang
Molecular Diversity and Regulation of Renal Potassium Channels
Physiol Rev, January 1, 2005; 85(1): 319 - 371.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel
Renal Vacuolar H+-ATPase
Physiol Rev, October 1, 2004; 84(4): 1263 - 1314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. H. Boese, O. Aziz, N. L. Simmons, and M. A. Gray
Kinetics and regulation of a Ca2+-activated Cl- conductance in mouse renal inner medullary collecting duct cells
Am J Physiol Renal Physiol, April 1, 2004; 286(4): F682 - F692.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Z. Qu, R. W. Wei, and H. C. Hartzell
Characterization of Ca2+-activated Cl- currents in mouse kidney inner medullary collecting duct cells
Am J Physiol Renal Physiol, August 1, 2003; 285(2): F326 - F335.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
G. Laverty, C. McWilliams, A. Sheldon, and S. S. Arnason
PTH stimulates a Cl--dependent and EIPA-sensitive current in chick proximal tubule cells in culture
Am J Physiol Renal Physiol, May 1, 2003; 284(5): F987 - F995.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
O. Devuyst and W. B. Guggino
Chloride channels in the kidney: lessons learned from knockout animals
Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1176 - F1191.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Wallace, M. Christensen, G. Reif, F. Belibi, B. Thrasher, D. Herrell, and J. J. Grantham
Electrolyte and fluid secretion by cultured human inner medullary collecting duct cells
Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1337 - F1350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-A. Konstas, M. Dabrowski, C. Korbmacher, and S. J. Tucker
Intrinsic Sensitivity of Kir1.1 (ROMK) to Glibenclamide in the Absence of SUR2B. IMPLICATIONS FOR THE IDENTITY OF THE RENAL ATP-REGULATED SECRETORY K+ CHANNEL
J. Biol. Chem., June 7, 2002; 277(24): 21346 - 21351.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Dong, J. Xu, C. G. Vanoye, R. Welch, G. G. MacGregor, G. Giebisch, and S. C. Hebert
An Amino Acid Triplet in the NH2 Terminus of Rat ROMK1 Determines Interaction with SUR2B
J. Biol. Chem., November 16, 2001; 276(47): 44347 - 44353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. P. Wallace, L. A. Rome, L. P. Sullivan, and J. J. Grantham
cAMP-dependent fluid secretion in rat inner medullary collecting ducts
Am J Physiol Renal Physiol, June 1, 2001; 280(6): F1019 - F1029.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Z. Bebok, A. Tousson, L. M. Schwiebert, and C. J. Venglarik
Improved oxygenation promotes CFTR maturation and trafficking in MDCK monolayers
Am J Physiol Cell Physiol, January 1, 2001; 280(1): C135 - C145.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. PERSU, O. DEVUYST, N. LANNOY, R. MATERNE, G. BROSNAHAN, P. A. GABOW, Y. PIRSON, and C. VERELLEN-DUMOULIN
CF Gene and Cystic Fibrosis Transmembrane Conductance Regulator Expression in Autosomal Dominant Polycystic Kidney Disease
J. Am. Soc. Nephrol., December 1, 2000; 11(12): 2285 - 2296.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Tanemoto, C. G. Vanoye, K. Dong, R. Welch, T. Abe, S. C. Hebert, and J. Z. Xu
Rat homolog of sulfonylurea receptor 2B determines glibenclamide sensitivity of ROMK2 in Xenopus laevis oocyte
Am J Physiol Renal Physiol, April 1, 2000; 278(4): F659 - F666.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yue, S. Devidas, and W. B. Guggino
The Two Halves of CFTR Form a Dual-pore Ion Channel
J. Biol. Chem., March 31, 2000; 275(14): 10030 - 10034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. R. Forte, R. M. London, R. H. Freeman, and W. J. Krause
Guanylin peptides: renal actions mediated by cyclic GMP
Am J Physiol Renal Physiol, February 1, 2000; 278(2): F180 - F191.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. K. Kishore, S. M. Ginns, C. M. Krane, S. Nielsen, and M. A. Knepper
Cellular localization of P2Y2 purinoceptor in rat renal inner medulla and lung
Am J Physiol Renal Physiol, January 1, 2000; 278(1): F43 - F51.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. M. QUINTON
Physiological Basis of Cystic Fibrosis: A Historical Perspective
Physiol Rev, January 1, 1999; 79(1): 3 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. N. SHEPPARD and M. J. WELSH
Structure and Function of the CFTR Chloride Channel
Physiol Rev, January 1, 1999; 79(1): 23 - 45.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. M. SCHWIEBERT, D. J. BENOS, M. E. EGAN, M. J. STUTTS, and W. B. GUGGINO
CFTR Is a Conductance Regulator as well as a Chloride Channel
Physiol Rev, January 1, 1999; 79(1): 145 - 166.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Devidas, H. Yue, and W. B. Guggino
The Second Half of the Cystic Fibrosis Transmembrane Conductance Regulator Forms a Functional Chloride Channel
J. Biol. Chem., November 6, 1998; 273(45): 29373 - 29380.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Rubera, M. Tauc, M. Bidet, C. Poujeol, B. Cuiller, A. Watrin, N. Touret, and P. Poujeol
Chloride currents in primary cultures of rabbit proximal and distal convoluted tubules
Am J Physiol Renal Physiol, November 1, 1998; 275(5): F651 - F663.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Ruknudin, D. H. Schulze, S. K. Sullivan, W. J. Lederer, and P. A. Welling
Novel Subunit Composition of a Renal Epithelial KATP Channel
J. Biol. Chem., June 5, 1998; 273(23): 14165 - 14171.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. M. Tulk and J. C. Edwards
NCC27, a homolog of intracellular Cl- channel p64, is expressed in brush border of renal proximal tubule
Am J Physiol Renal Physiol, June 1, 1998; 274(6): F1140 - F1149.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. M. Schwiebert, M. M. Morales, S. Devidas, M. E. Egan, and W. B. Guggino
Chloride channel and chloride conductance regulator domains of CFTR, the cystic fibrosis transmembrane conductance regulator
PNAS, March 3, 1998; 95(5): 2674 - 2679.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. D. Schultz, A. Takahashi, C. Liu, R. A. Frizzell, and M. Howard
FLAG epitope positioned in an external loop preserves normal biophysical properties of CFTR
Am J Physiol Cell Physiol, December 1, 1997; 273(6): C2080 - C2089.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Schmidt-Rose and T. J. Jentsch
Reconstitution of Functional Voltage-gated Chloride Channels from Complementary Fragments of CLC-1
J. Biol. Chem., August 15, 1997; 272(33): 20515 - 20521.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Cahill, M. W. Nason Jr., C. Ambrose, T.-Y. Yao, P. Thomas, and M. E. Egan
Identification of the Cystic Fibrosis Transmembrane Conductance Regulator Domains That Are Important for Interactions with ROMK2
J. Biol. Chem., May 26, 2000; 275(22): 16697 - 16701.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online