AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 270: F960-F970, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miralem, T.
Right arrow Articles by Templeton, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miralem, T.
Right arrow Articles by Templeton, D. M.

AJP - Renal Physiology, Vol 270, Issue 6 960-F970, Copyright © 1996 by American Physiological Society

ARTICLES

Collagen type I enhances endothelin-mediated contraction and induces nonproliferating phenotype in mesangial cells

T. Miralem, C. I. Whiteside and D. M. Templeton
Department of Clinical Biochemistry, University of Toronto, Ontario, Canada.

Accumulation of glomerular extracellular matrix is a characteristic accompaniment of mesangial cell proliferation in progressive renal disease. We examined how growth on several matrices affected the proliferative phenotype of cultured rat mesangial cells. Compared with growth on plastic, Matrigel, or mesangial matrix, collagen type I caused a decreased cell number at 72 h, decreased total DNA per culture, and a decrease in the incorporation of [3H]thymidine during S phase in cells released from quiescence. These antiproliferative and antimitogenic effects of collagen type I required growth on a collagen gel; soluble collagen or collagen fragments were without effect. Because a number of agents elicit both proliferative and contractile responses in mesangial cells, we examined the effect of growth on collagen on contractility. Compared with plastic, cells grown on collagen type I were more contractile, showed a higher Ca2+ signal in response to endothelin, and responded to endothelin with a more rapid myosin light-chain kinase-dependent phosphorylation of myosin light chain. We conclude that growth on a collagen type I gel uncouples contractility from a proliferative response in mesangial cells, suppressing proliferation while enhancing contraction and Ca2+ signaling in response to endothelin.


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
T. Miralem and H. K. Avraham
Extracellular Matrix Enhances Heregulin-Dependent BRCA1 Phosphorylation and Suppresses BRCA1 Expression through Its C Terminus
Mol. Cell. Biol., January 15, 2003; 23(2): 579 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T. Miralem and D. M. Templeton
Inactivation of kinase cascades in mesangial cells grown on collagen type I
Am J Physiol Renal Physiol, October 1, 1998; 275(4): F585 - F594.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. A. Dlugosz, S. Munk, X. Zhou, and C. I. Whiteside
Endothelin-1-induced mesangial cell contraction involves activation of protein kinase C-alpha , -delta , and -epsilon
Am J Physiol Renal Physiol, September 1, 1998; 275(3): F423 - F432.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. D. STOCKAND and S. C. SANSOM
Glomerular Mesangial Cells: Electrophysiology and Regulation of Contraction
Physiol Rev, July 1, 1998; 78(3): 723 - 744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online