AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 271: F30-F36, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bonilla-Felix, M.
Right arrow Articles by Jiang, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bonilla-Felix, M.
Right arrow Articles by Jiang, W.

AJP - Renal Physiology, Vol 271, Issue 1 30-F36, Copyright © 1996 by American Physiological Society

ARTICLES

Expression and localization of prostaglandin EP3 receptor mRNA in the immature rabbit kidney

M. Bonilla-Felix and W. Jiang
Department of Pediatrics, University of Texas-Health Science Center at Houston 77030, USA.

Arginine vasopressin (AVP)-stimulated cAMP generation is decreased in the immature collecting duct (CD). This is the result of prostaglandin antagonism, most likely via the inhibitory guanine nucleotide-binding protein (Gi). The EP3-subtype prostaglandin E2 (PGE2) receptor, which is coupled to Gi, could mediate this effect. We studied the developmental expression of EP3 receptor in the rabbit kidney. Higher levels of EP3 mRNA were observed in the immature kidney using three different assays: 1) reverse transcription-polymerase chain reaction (RT-PCR) with internal standard, 2) competitive PCR, and 3) ribonuclease protection assay. The highest levels were observed at 2 wk of age. RT-PCR from isolated nephron segments detected EP3 mRNA in the medullary thick ascending limb, cortical CD (CCD), and inner medullary CD (IMCD) of adult and immature kidneys. We conclude that 1) renal expression of EP3 mRNA is increased in immature kidneys and 2) EP3 mRNA is localized in the distal nephron. This suggests that EP3 receptor may play a role in the regulation of distal tubular transport during development.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Machida, S. Wakamatsu, Y. Izumi, T. Yosifovska, T. Matsuzaki, Y. Nakayama, Y. Kohda, T. Inoue, H. Saito, K. Tomita, et al.
Downregulation of the V2 vasopressin receptor in dehydration: mechanisms and role of renal prostaglandin synthesis
Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1274 - F1282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Bonilla-Felix
Development of water transport in the collecting duct
Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1093 - F1101.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
L. Tang, K. Loutzenhiser, and R. Loutzenhiser
Biphasic Actions of Prostaglandin E2 on the Renal Afferent Arteriole : Role of EP3 and EP4 Receptors
Circ. Res., March 31, 2000; 86(6): 663 - 670.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online