AJP - Renal AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 271: F1239-F1247, 1996;
0363-6127/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhu, Z.
Right arrow Articles by Arendshorst, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhu, Z.
Right arrow Articles by Arendshorst, W. J.

AJP - Renal Physiology, Vol 271, Issue 6 1239-F1247, Copyright © 1996 by American Physiological Society

ARTICLES

Angiotensin II-receptor stimulation of cytosolic calcium concentration in cultured renal resistance arterioles

Z. Zhu and W. J. Arendshorst
Department of Physiology, University of North Carolina at Chapel Hill 27599-7545, USA.

This study provides an initial characterization of basic morphological properties of cultures of vascular smooth muscle cells (VSMC) from rat preglomerular resistance vessels and of the functional coupling of angiotensin II (ANG II) receptors to cytosolic free calcium concentration ([Ca2+]i (fura 2 fluorescence photometry). Renal VSMC were isolated from interlobular arteries and afferent arterioles (< 50 microns) using an iron oxide sieving method and compared with rat aortic VSMC cultured under similar conditions. Quiescent monolayers maintained uniform morphology and [Ca2+]i signaling profile between passages 3 and 10. Arteriolar and aortic VSMC were spindle shaped and expressed smooth muscle-specific alpha-actin and myosin heavy chains SM-1 and SM-2. ANG II caused a rapid increase in [Ca2+]i, followed by a sustained plateau phase at 50-60% of the peak value. The initial maximum [Ca2+]i responses were dose dependent and of similar magnitude in renal arteriolar and aortic VSMC. ANG II (10(-7) M) increased [Ca2+]i from 50 to 240 nM in arteriolar and from 57 to 201 nM in aortic VSMC (P < 0.001 for both). Inhibition of ANG II effects on [Ca2+]i revealed significant signaling through distinct AT-receptor subtypes (losartan and PD-123319 sensitive) in renal arteriolar VSMC. In contrast, only losartan was effective in aortic VSMC. The AT2-receptor ligand CGP-42112 had no effect in either vessel type. Our results demonstrate that cultured arteriolar VSMC have anatomical similarities to aortic VSMC and functional differences in AT-receptor signaling in response to ANG II. This novel preparation should provide a useful approach with which to investigate cellular mechanisms concerning receptor coupling to signaling pathways involved in vascular reactivity of arteriolar VSMC in the microcirculation in general and the kidney in particular.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
X. C. Li, D. J. Campbell, M. Ohishi, S. Yuan, and J. L. Zhuo
AT1 receptor-activated signaling mediates angiotensin IV-induced renal cortical vasoconstriction in rats
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1024 - F1033.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. S. Wilcox
Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension?
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Sharma, L. Deelman, M. Madesh, B. Kurz, E. Ciccone, S. Siva, T. Hu, Y. Zhu, L. Wang, R. Henning, et al.
Involvement of transforming growth factor-{beta} in regulation of calcium transients in diabetic vascular smooth muscle cells
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1258 - F1270.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Cell Physiol.Home page
W.-L. Chan, N.-H. Holstein-Rathlou, and K.-P. Yip
Integrin mobilizes intracellular Ca2+ in renal vascular smooth muscle cells
Am J Physiol Cell Physiol, March 1, 2001; 280(3): C593 - C603.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. E. PURDY and W. J. ARENDSHORST
Iloprost Inhibits Inositol-1,4,5-Trisphosphate-Mediated Calcium Mobilization Stimulated by Angiotensin II in Cultured Preglomerular Vascular Smooth Muscle Cells
J. Am. Soc. Nephrol., January 1, 2001; 12(1): 19 - 28.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. E. Purdy and W. J. Arendshorst
EP1 and EP4 receptors mediate prostaglandin E2 actions in the microcirculation of rat kidney
Am J Physiol Renal Physiol, October 1, 2000; 279(4): F755 - F764.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. E. Purdy and W. J. Arendshorst
Prostaglandins buffer ANG II-mediated increases in cytosolic calcium in preglomerular VSMC
Am J Physiol Renal Physiol, December 1, 1999; 277(6): F850 - F858.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. K. Fellner and W. J. Arendshorst
Capacitative calcium entry in smooth muscle cells from preglomerular vessels
Am J Physiol Renal Physiol, October 1, 1999; 277(4): F533 - F542.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. M. Iversen and W. J. Arendshorst
Exaggerated Ca2+ signaling in preglomerular arteriolar smooth muscle cells of genetically hypertensive rats
Am J Physiol Renal Physiol, February 1, 1999; 276(2): F260 - F270.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online