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Am J Physiol Renal Physiol 273: F136-F143, 1997;
0363-6127/97 $5.00
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AJP - Renal Physiology, Vol 273, Issue 1 136-F143, Copyright © 1997 by American Physiological Society

ARTICLES

Cysteine S-conjugates activate transcription factor NF-kappa B in cultured renal epithelial cells

M. A. Otieno and M. W. Anders
University of Rochester, School of Medicine and Dentistry, Department of Pharmacology and Physiology, New York 14642, USA.

Activation of NF-kappa B by the nephrotoxic and cytotoxic cysteine S-conjugate S-(1,2-dichlorovinyl)-L-cysteine (DCVC) was investigated in porcine kidney-derived LLC-PK1 cells. DCVC induced binding of nuclear proteins to an NF-kappa B consensus oligonucleotide from the immunoglobulin kappa-light chain enhancer region, as determined by electrophoretic mobility shift assays, and the activated proteins were identified as the p50/RelA heterodimeric complex of NF-kappa B. Transient transfection experiments with a kappa B-controlled luciferase reporter construct showed that the NF-kappa B complex activated by DCVC was transcriptionally active. NF-kappa B transactivation was blocked by inhibition of DCVC bioactivation with the cysteine conjugate beta-lyase inhibitor (aminooxy)acetic acid, by the antioxidants N,N'-diphenyl-p-phenylenediamine and N-acetyl-L-cysteine, and by the protein kinase inhibitor staurosporine. The cysteine S-conjugates S-(2-bromo-2-chloro-1,1-difluoroethyl)-L-cysteine and S-(2-chloro-1,1,2-trifluoroethyl)-L-cysteine also activated NF-kappa B in LLC-PK1 cells. These results demonstrate that the NF-kappa B pathway is present in LLC-PK1 cells and is induced by cysteine S-conjugates. Inhibition of DCVC-induced transactivation of NF-kappa B by staurosporine and by antioxidants indicate roles for protein kinases and oxidative stress in the NF-kappa B pathway.


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G. K. Rangan, Y. Wang, Y.-C. Tay, and D. C. H. Harris
Inhibition of NFkappa B activation with antioxidants is correlated with reduced cytokine transcription in PTC
Am J Physiol Renal Physiol, November 1, 1999; 277(5): F779 - F789.
[Abstract] [Full Text] [PDF]


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