| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Renal Physiology, Vol 273, Issue 2 213-F223, Copyright © 1997 by American Physiological Society
ARTICLES |
J. Frokiaer, B. M. Christensen, D. Marples, J. C. Djurhuus, U. B. Jensen, M. A. Knepper and S. Nielsen
Department of Clinical Physiology, Aarhus University Hospital, Denmark.
In bilateral ureteral obstruction, both aquaporin-2 (AQP2) levels and urinary concentrating capacity are markedly reduced. However, the mechanisms involved in AQP2 downregulation are unknown. In rats with unilateral ureteral obstruction (UUO) the relative role of intrarenal and systemic factors can be evaluated. Semiquantitative immunoblotting revealed a marked decrease in AQP2 in obstructed kidneys to 23 +/- 7% (n = 9) of sham levels. This downregulation persisted 24 h after release of UUO. Furthermore, there was a significant but less extensive downregulation of AQP2 in the nonobstructed kidneys to 75 +/- 7% (n = 9) of sham levels. Consistent with impairment of collecting duct water reabsorption, free water clearance was greatly elevated in the obstructed kidneys (-2 +/- 1 microliter-min-1.kg-1, determined immediately after release) and only moderately elevated in nonobstructed kidneys (-44 +/- 5 microliters.min-1.kg-1) compared with sham-operated controls (-59 +/- 3 microliters.min-1.kg-1). Also AQP2 mRNA levels were reduced in obstructed kidneys. Immunocytochemistry confirmed the marked decrease in AQP2 expression in obstructed kidneys. In nonobstructed kidneys AQP2 was predominantly found in intracellular vesicles, which together with the reduced expression and elevated free water clearance strongly suggests a role of AQP2 in the observed compensatory diuresis from nonobstructed kidneys. The much lower AQP2 protein and mRNA levels in obstructed vs. nonobstructed kidneys are consistent with intrarenal factors playing a major role for downregulation of AQP2.
This article has been cited by other articles:
|
|
 |
|
  C. Li, W. Wang, R. Norregaard, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of epithelial sodium channel in rats with bilateral or unilateral ureteral obstruction Am J Physiol Renal Physiol, July 1, 2007; 293(1): F333 - F341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. O. Topcu, M. Pedersen, R. Norregaard, G. Wang, M. Knepper, J. C. Djurhuus, S. Nielsen, T. M. Jorgensen, and J. Frokiaer Candesartan prevents long-term impairment of renal function in response to neonatal partial unilateral ureteral obstruction Am J Physiol Renal Physiol, February 1, 2007; 292(2): F736 - F748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Schrier Body Water Homeostasis: Clinical Disorders of Urinary Dilution and Concentration J. Am. Soc. Nephrol., July 1, 2006; 17(7): 1820 - 1832. [Full Text] [PDF]
|
|
|
|
|
|
C. Li, Y. Shi, W. Wang, C. Sardeli, T.-H. Kwon, K. Thomsen, T. Jonassen, J. C. Djurhuus, M. A. Knepper, S. Nielsen, et al. {alpha}-MSH prevents impairment in renal function and dysregulation of AQPs and Na-K-ATPase in rats with bilateral ureteral obstruction Am J Physiol Renal Physiol, February 1, 2006; 290(2): F384 - F396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Hasler, S. Nielsen, E. Feraille, and P.-Y. Martin Posttranscriptional control of aquaporin-2 abundance by vasopressin in renal collecting duct principal cells Am J Physiol Renal Physiol, January 1, 2006; 290(1): F177 - F187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Norregaard, B. L. Jensen, C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiaer COX-2 inhibition prevents downregulation of key renal water and sodium transport proteins in response to bilateral ureteral obstruction Am J Physiol Renal Physiol, August 1, 2005; 289(2): F322 - F333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, M. Pedersen, C. Li, J. G. Wen, K. Thomsen, H. Stodkilde-Jorgensen, T. M. Jorgensen, M. A. Knepper, S. Nielsen, J. C. Djurhuus, et al. Early release of neonatal ureteral obstruction preserves renal function Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1087 - F1099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, J. D. Klein, W. Wang, M. A. Knepper, S. Nielsen, J. M. Sands, and J. Frokiaer Altered expression of urea transporters in response to ureteral obstruction Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1154 - F1162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. M. Christensen, D. Marples, Y.-H. Kim, W. Wang, J. Frokiaer, and S. Nielsen Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI Am J Physiol Cell Physiol, April 1, 2004; 286(4): C952 - C964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiaer Altered expression of major renal Na transporters in rats with bilateral ureteral obstruction and release of obstruction Am J Physiol Renal Physiol, November 1, 2003; 285(5): F889 - F901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, M. A. Knepper, S. Nielsen, and J. Frokiar Downregulation of renal aquaporins in response to unilateral ureteral obstruction Am J Physiol Renal Physiol, May 1, 2003; 284(5): F1066 - F1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, M. A. Knepper, S. Nielsen, and J. Frokiar Altered expression of major renal Na transporters in rats with unilateral ureteral obstruction Am J Physiol Renal Physiol, January 1, 2003; 284(1): F155 - F166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nielsen, J. Frokiar, D. Marples, T.-H. Kwon, P. Agre, and M. A. Knepper Aquaporins in the Kidney: From Molecules to Medicine Physiol Rev, January 1, 2002; 82(1): 205 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, W. Wang, T.-H. Kwon, L. Isikay, J. G. Wen, D. Marples, J. C. Djurhuus, A. Stockwell, M. A. Knepper, S. Nielsen, et al. Downregulation of AQP1, -2, and -3 after ureteral obstruction is associated with a long-term urine-concentrating defect Am J Physiol Renal Physiol, July 1, 2001; 281(1): F163 - F171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Frokiaer, P. Fernandez-Llama, M. A. Knepper, and S. Nielsen Reduced abundance of aquaporins in rats with bilateral ischemia-induced acute renal failure: prevention by alpha -MSH Am J Physiol Renal Physiol, September 1, 1999; 277(3): F413 - F427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. FERNÁNDEZ-LLAMA, P. ANDREWS, R. TURNER, S. SAGGI, J. DIMARI, T.-H. KWON, S. NIELSEN, R. SAFIRSTEIN, and M. A. KNEPPER Decreased Abundance of Collecting Duct Aquaporins in Post-Ischemic Renal Failure in Rats J. Am. Soc. Nephrol., August 1, 1999; 10(8): 1658 - 1668. [Abstract] [Full Text]
|
|
|
|
|
|
H. WEN, J. FRØKI&Aelig;R, T.-H. KWON, and S. NIELSEN Urinary Excretion of Aquaporin-2 in Rat Is Mediated by a Vasopressin-Dependent Apical Pathway J. Am. Soc. Nephrol., July 1, 1999; 10(7): 1416 - 1429. [Abstract] [Full Text]
|
|
|
|
|
|
S. NIELSEN, T.-H. KWON, B. M. CHRISTENSEN, D. PROMENEUR, J. FRØKI&Aelig;R, and D. MARPLES Physiology and Pathophysiology of Renal Aquaporins J. Am. Soc. Nephrol., March 1, 1999; 10(3): 647 - 663. [Abstract] [Full Text]
|
|
|
|
|
|
D. Marples, J. Frokiaer, and S. Nielsen Long-term regulation of aquaporins in the kidney Am J Physiol Renal Physiol, March 1, 1999; 276(3): F331 - F339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen Reduced AQP1, -2, and -3 levels in kidneys of rats with CRF induced by surgical reduction in renal mass Am J Physiol Renal Physiol, November 1, 1998; 275(5): F724 - F741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Marples, B. M. Christensen, J. Frokiaer, M. A. Knepper, and S. Nielsen Dehydration reverses vasopressin antagonist-induced diuresis and aquaporin-2 downregulation in rats Am J Physiol Renal Physiol, September 1, 1998; 275(3): F400 - F409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Christensen, D. Marples, U. B. Jensen, J. Frokiaer, D. Sheikh-Hamad, M. Knepper, and S. Nielsen Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution Am J Physiol Renal Physiol, August 1, 1998; 275(2): F285 - F297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Surendran, S. P. McCaul, and T. C. Simon A role for Wnt-4 in renal fibrosis Am J Physiol Renal Physiol, March 1, 2002; 282(3): F431 - F441. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
