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Am J Physiol Renal Physiol 277: F914-F925, 1999;
0363-6127/99 $5.00
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Vol. 277, Issue 6, F914-F925, December 1999

The rat Pkd2 protein assumes distinct subcellular distributions in different organs

Nicholas Obermüller1, A. Rachel Gallagher2, Yiqiang Cai3, Nikolaus Gassler4, Norbert Gretz1, Stefan Somlo3, and Ralph Witzgall2

1 Medical Research Center, Klinikum Mannheim, University of Heidelberg, 68167 Mannheim; 2 Institute for Anatomy and Cell Biology I, University of Heidelberg, 69120 Heidelberg, Germany; 3 Section of Nephrology, Yale University School of Medicine, New Haven, Connecticut 06520-8029; and 4 Department of Pathology, University of Heidelberg, 69120 Heidelberg, Germany

Mutations in the PKD2 gene account for ~15% of all cases of autosomal-dominant polycystic kidney disease. In the present study the cellular distribution of the Pkd2 protein was investigated by immunohistochemistry in different rat organs. Although the Pkd2 protein showed a widespread expression, a strikingly different distribution of the protein was observed between individual organs. Whereas in renal distal tubules and in striated ducts of salivary glands a basal-to-basolateral distribution of Pkd2 was found, a punctate cytoplasmic location was detected in the adrenal gland, ovary, cornea, and smooth muscle cells of blood vessels. Interestingly, in the adrenal gland and ovary, the rat Pkd2 protein was more heavily N-glycosylated than in the kidney and salivary gland. These results suggest that Pkd2 accomplishes its functions by interacting with proteins located in different cellular compartments. The extrarenal expression pattern of the Pkd2 protein hints at other candidate sites of disease manifestations in patients carrying PKD2 mutations.

polycystic kidney disease; immunohistochemistry; endoplasmic reticulum


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