AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 280: F61-F70, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hardiek, K.
Right arrow Articles by Deitrick, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hardiek, K.
Right arrow Articles by Deitrick, C.
Vol. 280, Issue 1, F61-F70, January 2001

Proximal tubule cell response to radiographic contrast media

Kathy Hardiek1,2, Richard E. Katholi1,2, Vickram Ramkumar1, and Cynthia Deitrick1,2

1 Department of Pharmacology, Southern Illinois University School of Medicine, Springfield 62794-9629; and 2 Prairie Education Research Cooperative, Springfield, Illinois 62701

Renal dysfunction associated with contrast media (CM) administration is generally attributed to reduced renal blood flow. Studies, however, also suggest direct tubular effects of CM, whose mechanisms remain unclear. This study was conducted to assess the chemotoxic effects of iopamidol, a prototypic CM, on a porcine proximal tubule (PT) cell line, LLC-PK1 cells. Results indicate that iopamidol did not affect cell viability (determined by trypan blue exclusion and fluorescein staining), but did reduce cell proliferation. Moreover, iopamidol altered mitochondrial function, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction and mitochondrial membrane potential. Decreased MTT reduction was evident with all CM tested, and its rapid recovery after CM removal suggests that inhibition of mitochondrial function is reversible. Injury to PT cells by iopamidol is supported by the fact that CM increase extracellular adenosine, an indicator of cellular stress. This study provides greater insight into the mechanism underlying the nephrotoxicity induced by contrast in patients and explains the reversibility of this toxicity.

adenosine; contrast media nephrotoxicity; iopamidol; LLC-PK1 cells; mitochondria


This article has been cited by other articles:


Home page
 RadiologyHome page
M. C. Heinrich, L. Haberle, V. Muller, W. Bautz, and M. Uder
Nephrotoxicity of Iso-osmolar Iodixanol Compared with Nonionic Low-osmolar Contrast Media: Meta-analysis of Randomized Controlled Trials
Radiology, January 1, 2009; 250(1): 68 - 86.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. T. Billings IV, S. W. C. Chen, M. Kim, S. W. Park, J. H. Song, S. Wang, J. Herman, V. D'Agati, and H. T. Lee
{alpha}2-Adrenergic agonists protect against radiocontrast-induced nephropathy in mice
Am J Physiol Renal Physiol, September 1, 2008; 295(3): F741 - F748.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
Y. Yokomaku, T. Sugimoto, S. Kume, S.-i. Araki, K. Isshiki, M. Chin-Kanasaki, M. Sakaguchi, N. Nitta, M. Haneda, D. Koya, et al.
Asialoerythropoietin Prevents Contrast-Induced Nephropathy
J. Am. Soc. Nephrol., February 1, 2008; 19(2): 321 - 328.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
E. Seeliger, B. Flemming, T. Wronski, M. Ladwig, K. Arakelyan, M. Godes, M. Mockel, and P. B. Persson
Viscosity of Contrast Media Perturbs Renal Hemodynamics
J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2912 - 2920.
[Abstract] [Full Text] [PDF]

Home page
 RadiologyHome page
M. C. Heinrich, M. K. Kuhlmann, S. Kohlbacher, M. Scheer, A. Grgic, M. B. Heckmann, and M. Uder
Cytotoxicity of Iodinated and Gadolinium-based Contrast Agents in Renal Tubular Cells at Angiographic Concentrations: In Vitro Study
Radiology, December 19, 2006; (2006) 2422060245.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. T. Lee, M. Jan, S. C. Bae, J. D. Joo, F. R. Goubaeva, J. Yang, and M. Kim
A1 adenosine receptor knockout mice are protected against acute radiocontrast nephropathy in vivo
Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1367 - F1375.
[Abstract] [Full Text] [PDF]

Home page
 RadiologyHome page
T. Skotland, D. Grant, M. Heinrich, and M. Uder
In Vitro Cytotoxic Effects of Iodinated Contrast Media on a Renal Tubular Cell Line
Radiology, February 1, 2006; 238(2): 758 - 760.
[Full Text] [PDF]

Home page
 RadiologyHome page
M. C. Heinrich, M. K. Kuhlmann, A. Grgic, M. Heckmann, B. Kramann, and M. Uder
Cytotoxic Effects of Ionic High-osmolar, Nonionic Monomeric, and Nonionic Iso-osmolar Dimeric Iodinated Contrast Media on Renal Tubular Cells in Vitro
Radiology, June 1, 2005; 235(3): 843 - 849.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. Yano, Y. Itoh, T. Kubota, T. Sendo, T. Koyama, T. Fujita, K. Saeki, A. Yuo, and R. Oishi
A Prostacyclin Analog Prevents Radiocontrast Nephropathy via Phosphorylation of Cyclic AMP Response Element Binding Protein
Am. J. Pathol., May 1, 2005; 166(5): 1333 - 1342.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online