AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 280: F278-F282, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Díez-Sampedro, A.
Right arrow Articles by Hirayama, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Díez-Sampedro, A.
Right arrow Articles by Hirayama, B. A.
Vol. 280, Issue 2, F278-F282, February 2001

Na+-to-sugar stoichiometry of SGLT3

Ana Díez-Sampedro, Sepehr Eskandari, Ernest M. Wright, and Bruce A. Hirayama

Department of Physiology, University of California Los Angeles, School of Medicine, Los Angeles, California 90095-1751

Sodium-glucose cotransporters (SGLTs) mediate active transport of sugar across cell membranes coupled to Na+, by using the electrochemical gradient as a driving force. In the kidney, there is evidence for two kinds of cotransporters, a high-affinity, low-capacity system, and a low-affinity, high-capacity system, with differences in substrate specificity and kinetics. Three renal SGLT clones have been identified: SGLT1 corresponding to the high-affinity system, and SGLT2 and SGLT3 with properties reminiscent of the low-affinity system. We have determined the stoichiometry of pig SGLT3 (pSGLT3) by using a direct method, comparing the substrate-induced inward charge to 22Na or [14C]alpha -methyl-D-glucopyranoside uptake in the same oocyte. pSGLT3 stoichiometry is 2 Na+:1 sugar, the same as that for SGLT1, but different from SGLT2 (1:1). The Na+ Hill coefficient for SGLT3 is ~1.5, suggesting low cooperativity between Na+ binding sites. Thus SGLT3 has functional characteristics intermediate between SGLT1 and SGLT2, so, whereas SGLT3 stoichiometry is the same as that for SGLT1 (2:1), sugar affinity and specificity are similar to SGLT2.

sodium-glucose cotransporter; electrophysiology; ion-substrate coupling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
C. Schmidt, K. Hocherl, and M. Bucher
Regulation of renal glucose transporters during severe inflammation
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F804 - F811.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
I. M. Larrayoz, A. Fernandez-Nistal, A. Garces, E. Gorraitz, and M. P. Lostao
Characterization of the rat Na+/nucleoside cotransporter 2 and transport of nucleoside-derived drugs using electrophysiological methods
Am J Physiol Cell Physiol, December 1, 2006; 291(6): C1395 - C1404.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. M. Smith, M. D. Slugoski, S. K. Loewen, A. M. L. Ng, S. Y. M. Yao, X.-Z. Chen, E. Karpinski, C. E. Cass, S. A. Baldwin, and J. D. Young
The Broadly Selective Human Na+/Nucleoside Cotransporter (hCNT3) Exhibits Novel Cation-coupled Nucleoside Transport Characteristics
J. Biol. Chem., July 8, 2005; 280(27): 25436 - 25449.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
F. Bourgeois, M. J Coady, and J.-Y. Lapointe
Determination of transport stoichiometry for two cation-coupled myo-inositol cotransporters: SMIT2 and HMIT
J. Physiol., March 1, 2005; 563(2): 333 - 343.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. Santer, M. Kinner, C. L. Lassen, R. Schneppenheim, P. Eggert, M. Bald, J. Brodehl, M. Daschner, J. H.H. Ehrich, M. Kemper, et al.
Molecular Analysis of the SGLT2 Gene in Patients with Renal Glucosuria
J. Am. Soc. Nephrol., November 1, 2003; 14(11): 2873 - 2882.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Y. Yao, A. M. Ng, S. K. Loewen, C. E. Cass, S. A. Baldwin, and J. D. Young
An ancient prevertebrate Na+-nucleoside cotransporter (hfCNT) from the Pacific hagfish (Eptatretus stouti)
Am J Physiol Cell Physiol, July 1, 2002; 283(1): C155 - C168.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online