| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
-subunit of the
epithelial sodium channel
1 Department of Medicine, University of Pittsburgh, Pittsburgh 15261; 2 Department of Medicine, Maine Medical Center, Portland, Maine 04102; 3 Department of Physiology, Emory University, Atlanta, Georgia 30322; 4 Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104; and 5 Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, Alabama 35294
The renal epithelial cell line A6,
derived from Xenopus laevis, expresses epithelial
Na+ channels (ENaCs) and serves as a model system to study
hormonal regulation and turnover of ENaCs. Our previous studies suggest that the 
-subunit of Xenopus ENaC (-xENaC)
is detectable as 150- and 180-kDa polypeptides, putative immature and
mature -subunit heterodimers. The 150- and 180-kDa
-xENaC were present in distinct fractions after
sedimentation of A6 cell lysate through a sucrose density gradient. Two
anti--xENaC antibodies directed against distinct domains
demonstrated that only 180-kDa -xENaC was expressed at
the apical cell surface. The half-life of cell surface-expressed -xENaC was 24-30 h, suggesting that once ENaC
matures and is expressed at the plasma membrane, its turnover is
similar to that reported for mature cystic fibrosis transmembrane
conductance regulator. No significant changes in apical surface
expression of -xENaC were observed after treatment of A6
cells with aldosterone for 24 h, despite a 5.3-fold increase in
short-circuit current. This lack of change in surface expression is
consistent with previous observations in A6 cells and suggests that
aldosterone regulates ENaC gating and increases channel open probability.
epithelial sodium transport; epithelial sodium channels; ion channel; renal epithelium
This article has been cited by other articles:
|
|
 |
|
  V. Bhalla, R. Soundararajan, A. C. Pao, H. Li, and D. Pearce Disinhibitory pathways for control of sodium transport: regulation of ENaC by SGK1 and GILZ Am J Physiol Renal Physiol, October 1, 2006; 291(4): F714 - F721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Malik, S. R. Price, W. E. Mitch, Q. Yue, and D. C. Eaton Regulation of epithelial sodium channels by the ubiquitin-proteasome proteolytic pathway Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1285 - F1294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and F. Verrey Impact of Nedd4 Proteins and Serum and Glucocorticoid-Induced Kinases on Epithelial Na+ Transport in the Distal Nephron J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3167 - 3174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Adebamiro, Y. Cheng, J. P. Johnson, and R. J. Bridges Endogenous Protease Activation of ENaC: Effect of Serine Protease Inhibition on ENaC Single Channel Properties J. Gen. Physiol., September 26, 2005; 126(4): 339 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Malik, Q. Yue, G. Yue, X. J. Chen, S. R. Price, W. E. Mitch, and D. C. Eaton Role of Nedd4-2 and polyubiquitination in epithelial sodium channel degradation in untransfected renal A6 cells expressing endogenous ENaC subunits Am J Physiol Renal Physiol, July 1, 2005; 289(1): F107 - F116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Hardiman, C. M. McNicholas-Bevensee, J. Fortenberry, C. T. Myles, B. Malik, D. C. Eaton, and S. Matalon Regulation of Amiloride-Sensitive Na+ Transport by Basal Nitric Oxide Am. J. Respir. Cell Mol. Biol., May 1, 2004; 30(5): 720 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. A. Weisz and J. P. Johnson Noncoordinate regulation of ENaC: paradigm lost? Am J Physiol Renal Physiol, November 1, 2003; 285(5): F833 - F842. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lebowitz, B. An, R. S. Edinger, M. L. Zeidel, and J. P. Johnson Effect of altered Na+ entry on expression of apical and basolateral transport proteins in A6 epithelia Am J Physiol Renal Physiol, September 1, 2003; 285(3): F524 - F531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Booth and J. D. Stockand Targeted degradation of ENaC in response to PKC activation of the ERK1/2 cascade Am J Physiol Renal Physiol, May 1, 2003; 284(5): F938 - F947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Shlyonsky, F. Mies, and S. Sariban-Sohraby Epithelial sodium channel activity in detergent-resistant membrane microdomains Am J Physiol Renal Physiol, January 1, 2003; 284(1): F182 - F188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Becchetti, B. Malik, G. Yue, P. Duchatelle, O. Al-Khalili, T. R. Kleyman, and D. C. Eaton Phosphatase inhibitors increase the open probability of ENaC in A6 cells Am J Physiol Renal Physiol, November 1, 2002; 283(5): F1030 - F1045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Hill, B. An, and J. P. Johnson Endogenously Expressed Epithelial Sodium Channel Is Present in Lipid Rafts in A6 Cells J. Biol. Chem., September 6, 2002; 277(37): 33541 - 33544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hanwell, T. Ishikawa, R. Saleki, and D. Rotin Trafficking and Cell Surface Stability of the Epithelial Na+ Channel Expressed in Epithelial Madin-Darby Canine Kidney Cells J. Biol. Chem., March 15, 2002; 277(12): 9772 - 9779. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
