AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 281: F318-F325, 2001;
0363-6127/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fejes-Tóth, G.
Right arrow Articles by Náray-Fejes-Tóth, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fejes-Tóth, G.
Right arrow Articles by Náray-Fejes-Tóth, A.
Vol. 281, Issue 2, F318-F325, August 2001

Immunohistochemical localization of colonic H-K-ATPase to the apical membrane of connecting tubule cells

Géza Fejes-Tóth and Anikó Náray-Fejes-Tóth

Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756

Previous studies indicate that the colonic H-K-ATPase mRNA is expressed as the distal nephron. However, the exact intrarenal localization of the colonic H-K-ATPase protein is still unclear. The goal of the present study was to determine the cellular and subcellular localization of the colonic H-K-ATPase protein in the rabbit kidney. We used three monoclonal antibodies (MAbs) directed against different epitopes of the rabbit colonic H-K-ATPase alpha -subunit (HKalpha 2) to localize HKalpha 2 protein by immunofluorescence labeling of kidney sections and laser-scanning confocal microscopy. The specificity of the MAbs was confirmed by reaction with a single ~100-kDa band on Western blots of distal colon. Specific immunohistochemical reaction with the apical membrane of surface epithelial cells was observed with all three MAbs on distal colon sections. In rabbit kidney, immunofluorescence was detected only on the apical membrane of connecting tubule cells. Immunofluorescence was not detected in the cortical-, outer-, and inner-medullary collecting ducts. Furthermore, costaining with principal- and intercalated cell-specific MAbs and a MAb against the thick ascending limb suggests that these cell types express HKalpha 2 protein at levels that are below the detection limit with this method. We conclude that in the rabbit kidney, under normal dietary conditions, the HKalpha 2 protein is expressed in the apical membrane of connecting tubule cells.

potassium transport; confocal microscopy; rabbit kidney


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
N. B. Pestov, T. V. Korneenko, M. I. Shakhparonov, G. E. Shull, and N. N. Modyanov
Loss of acidification of anterior prostate fluids in Atp12a-null mutant mice indicates that nongastric H-K-ATPase functions as proton pump in vivo
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C366 - C374.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel
Renal Vacuolar H+-ATPase
Physiol Rev, October 1, 2004; 84(4): 1263 - 1314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. B. Pestov, T. V. Korneenko, R. Radkov, H. Zhao, M. I. Shakhparonov, and N. N. Modyanov
Identification of the {beta}-subunit for nongastric H-K-ATPase in rat anterior prostate
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1229 - C1237.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. N. Helms, G. Fejes-Toth, and A. Naray-Fejes-Toth
Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression
Am J Physiol Renal Physiol, March 1, 2003; 284(3): F480 - F487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Crambert, J.-D. Horisberger, N. N. Modyanov, and K. Geering
Human nongastric H+-K+-ATPase: transport properties of ATP1al1 assembled with different beta -subunits
Am J Physiol Cell Physiol, July 1, 2002; 283(1): C305 - C314.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. B. Pestov, T. V. Korneenko, G. Adams, M. Tillekeratne, M. I. Shakhparonov, and N. N. Modyanov
Nongastric H-K-ATPase in rodent prostate: lobe-specific expression and apical localization
Am J Physiol Cell Physiol, April 1, 2002; 282(4): C907 - C916.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online