AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F509-F516, 2002. First published April 30, 2002; doi:10.1152/ajprenal.00236.2001
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/3/F509    most recent
00236.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, M.-Z.
Right arrow Articles by McKanna, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, M.-Z.
Right arrow Articles by McKanna, J. A.
Vol. 283, Issue 3, F509-F516, September 2002

Mineralocorticoid regulation of cyclooxygenase-2 expression in rat renal medulla

Ming-Zhi Zhang1, Chuan-Ming Hao2, Matthew D. Breyer2, Raymond C. Harris2, and James A. McKanna1

George O'Brien Center for Kidney and Urologic Diseases and Departments of 1 Cell Biology and 2 Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

The renal inner medulla and its distal one-third, the papilla, are major sites of prostanoid synthesis involved in water and electrolyte homeostasis. These sites contain variable levels of cyclooxygenase (COX)-2, a key prostaglandin synthase enzyme that is sensitive to adrenal steroids. Immunoreactive renal medullary COX-2, restricted to interstitial cells in control adult rats, shows a gradient of intense staining at the tip of the papilla that gradually diminishes to undetectable levels in the proximal inner medulla. We used adrenalectomy (ADX) and steroid replacement to investigate the effects of steroids on papillary COX-2. Immunoblots demonstrate that papillary COX-2 was reduced by one-half after 2 wk ADX; glucocorticoid replacement ameliorated the decline but not to control levels. Mineralocorticoid (deoxycorticosterone acetate; DOCA) replacement stimulated papillary COX-2 more than fivefold over control; both the intensity of immunostaining and the numbers of COX-2-positive cells in the inner medulla increased. Similar stimulation of papillary COX-2 resulted from DOCA treatment of normal control rats, but the response was blunted in rats fed a low-salt diet and absent in Brattleboro rats. DOCA treatment of mouse renal medullary interstitial cells in culture had no effect, but increased tonicity of the culture medium with NaCl caused strong upregulation of COX-2. Urea, a permeant molecule, had no effect. Together, these results suggest that mineralocorticoids lead to upregulation of COX-2 in rat renal medulla by indirect pathways, probably involving induced electrolyte hypertonicity in the interstitial fluid.

adrenal steroid; prostaglandin G/H synthase; papilla; deoxycorticosterone acetate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. A. Brennan, S. Kaufman, S. W. Reynolds, B. T. McCook, G. Kan, I. Christiaens, M. E. Symonds, and D. M. Olson
Differential effects of maternal nutrient restriction through pregnancy on kidney development and later blood pressure control in the resulting offspring
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2008; 295(1): R197 - R205.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
B. Yao, J. Xu, R. C. Harris, and M.-Z. Zhang
Renal localization and regulation of 15-hydroxyprostaglandin dehydrogenase
Am J Physiol Renal Physiol, February 1, 2008; 294(2): F433 - F439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. Yao, R. C. Harris, and M.-Z. Zhang
Interactions between 11{beta}-hydroxysteroid dehydrogenase and COX-2 in kidney
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1767 - R1773.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Chen, M. Zhao, R. Rao, H. Inoue, and C.-M. Hao
C/EBP{beta} and Its Binding Element Are Required for NF{kappa}B-induced COX2 Expression Following Hypertonic Stress
J. Biol. Chem., April 22, 2005; 280(16): 16354 - 16359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M.-Z. Zhang, B. Yao, J. A. McKanna, and R. C. Harris
Cross talk between the intrarenal dopaminergic and cyclooxygenase-2 systems
Am J Physiol Renal Physiol, April 1, 2005; 288(4): F840 - F845.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Madsen, J. Stubbe, T. Yang, O. Skott, S. Bachmann, and B. L. Jensen
Low endogenous glucocorticoid allows induction of kidney cortical cyclooxygenase-2 during postnatal rat development
Am J Physiol Renal Physiol, January 1, 2004; 286(1): F26 - F37.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M.-Z. Zhang, S.-w. Wang, H. Cheng, Y. Zhang, J. A. McKanna, and R. C. Harris
Regulation of renal cortical cyclooxygenase-2 in young rats
Am J Physiol Renal Physiol, November 1, 2003; 285(5): F881 - F888.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. Campean, F. Theilig, A. Paliege, M. Breyer, and S. Bachmann
Key enzymes for renal prostaglandin synthesis: site-specific expression in rodent kidney (rat, mouse)
Am J Physiol Renal Physiol, July 1, 2003; 285(1): F19 - F32.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online