AJP - Renal Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 283: F689-F698, 2002. First published May 7, 2002; doi:10.1152/ajprenal.00020.2002
0363-6127/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/4/F689    most recent
00020.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nasrallah, R.
Right arrow Articles by Hébert, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nasrallah, R.
Right arrow Articles by Hébert, R. L.
Vol. 283, Issue 4, F689-F698, October 2002

Localization of IP in rabbit kidney and functional role of the PGI2/IP system in cortical collecting duct

Rania Nasrallah1, Rolf M. Nusing2, and Richard L. Hébert1

1 Department of Cellular and Molecular Medicine, Kidney Research Centre, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5; and 2 Department of Experimental Pediatrics and Clinical Pharmacology, Faculty of Medicine, Philipps University of Marburg, 35033 Marburg, Germany

To clarify the role of the PGI2/PGI2 receptor (IP) system in rabbit cortical collecting duct (RCCD), we characterized the expression of IP receptors in the rabbit kidney. We show by Northern and Western blotting that IP mRNA and protein was detectable in all three regions of the kidney. To determine how PGI2 signals, we compared the effects of different PGI2 analogs [iloprost (ILP), carba-prostacyclin (c-PGI2), and cicaprost (CCP)] in the isolated perfused RCCD. PGI2 analogs did not increase water flow (Lp). Although PGI2 analogs did not reduce an established Lp response to 8-chlorophenylthio-cAMP, they equipotently inhibited AVP-stimulated Lp by 45%. The inhibitory effect of ILP and c-PGI2 on AVP-stimulated Lp is partially reversed by the protein kinase C inhibitor staurosporine and abolished by pertussis toxin; no effect was obtained with CCP. In fura 2-loaded RCCD, CCP did not alter cytosolic Ca2+ concentration ([Ca2+]i), but, in the presence of CCP, individual infusion of ILP and PGE2 increased [Ca2+]i, suggesting that CCP did not cause desensitization to either ILP or PGE2. We concluded that ILP and c-PGI2 activate PKC and the liberation of [Ca2+]i but not CCP. This suggested an important role for phosphatidylinositol hydrolysis in mediating ILP and c-PGI2 effects but not CCP in RCCD.

cortical collecting duct; intracellular calcium; prostaglandin I2; transepithelial voltage; vasopressin; water transport


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Nasrallah and R. L. Hebert
Prostacyclin signaling in the kidney: implications for health and disease
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F235 - F246.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Nasrallah and R. L. Hebert
Reduced IP receptors in STZ-induced diabetic rat kidneys and high-glucose-treated mesangial cells
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F673 - F681.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online