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![]() [Cover Caption] Other Issues: |
Contents: Volume 285, Issue 3
September 2003 [Index by Author] [Editorial Board]
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Cover: A two-photon fluorescence intravital image of the renal cortex. A 500,000-Da FITC-labeled dextran (green), 3,000-Da rhodamine-labeled dextran (red), and the nuclear stain Hoechst 33342 (blue) were injected via the tail vein into a rat after 48 h of reperfusion after 45 min of renal ischemia. The large FITC-labeled dextran was generally retained in the vascular space and identifies the microvasculature. Signal voids in the vascular space are due to the relative rapid movement of cellular elements (red and white blood cells) compared with the speed of image acquisition. Proximal tubules are identified by the red punctate appearance due to the internalization of the small rhodamine-labeled dextran. Note the area of extravasation of both the small and large dextrans from the vascular space into the interstitium after ischemia. The extent of extravasation peaked at 4 h of reperfusion and returned to close to normal after 48 h of reperfusion. See related article by Sutton TA, Mang HE, Campos SB, Sandoval RM, Yoder MC, and Molitoris BA. Injury of the renal microvascular endothelium alters barrier function after ischemia. Am J Physiol Renal Physiol 285: F191-F198, 2003.
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