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 [Cover Caption] Other Issues: 
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Contents: Volume 294, Issue 6
June 2008 [Index by Author] [Editorial Board]
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= article is free immediately upon publication
(all articles are free one year after publication)
Cover: Lysosomal permeability after incubation with glycated collagen as assessed by decreased co-localization of dextran-Texas red with Lamp-1. Human umbilical vein endothelial cells (HUVEC) were incubated for 24 h in culture medium containing dextran conjugated to Texas red (MW 10,000) followed by a 24-h chase in a dextran-free culture medium for accumulation in lysosomes. Cells were incubated with glycated collagen (GC), native/glycated collagen (1:3 GC), native collagen (NC), or remained untreated (control) and studied at the indicated times. Displayed is a cell treated with chloroquine (300 mM), which was used as a positive control. Cells were fixed, permeabilized, and stained with antibody to Lamp-1 and a nuclear stain, Hoechst 33342. Cells were imaged using a Nikon TE-2000U microscope equipped with a Spot Insight camera (Diagnostic Instruments). See related article by Patschan SA, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, and Goligorsky MS. Mapping mechanisms and charting the time course of premature senescence and apoptosis: lysosomal dysfunction and gangioside accumulation in endothelial cells. Am J Physiol Renal Physiol. 294: F100–F110, 2008.
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